Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Arie Ben-Bassat"'
Autor:
Robert DiCosimo, F. Sima Sariaslani, Lixuan Huang, Arie Ben-Bassat, Carl E. Camp, Grace A. Crum, Robert J Trotman, Sharon L. Haynie
Publikováno v:
Biotechnology Progress. 23:638-644
An Escherichia coli catalyst with tyrosine ammonia lyase activity (TAL) has been stabilized for repeated use in batch conversions of high tyrosine solids to p-hydroxycinnamic acid (pHCA). The TAL biocatalyst was stabilized by controlling the reaction
Autor:
Matthew A. Plummer, Robert DiCosimo, Amy E. Sigmund, Alison M. Walls, Arie Ben-Bassat, William L. Spillan
Publikováno v:
Advanced Synthesis & Catalysis. 350:1761-1769
A chemoenzymatic process has been developed that employs an immobilized microbial nitrilase biocatalyst for the conversion of glycolonitrile to high-purity glycolic acid. The specific activity of this immobilized cell biocatalyst decreased significan
Autor:
Zhixiong Xue, Michael McCluskey, F. Sima Sariaslani, Arie Ben-Bassat, Lixuan Huang, Keith Cantera
Publikováno v:
Enzyme and Microbial Technology. 42:58-64
p -Hydroxycinnamic acid (pHCA), which serves as the starting material for production of a number of industrial chemicals, can be produced by deamination of tyrosine by phenylalanine/tyrosine ammonia lyase (PAL/TAL) enzyme. In this study, we character
Autor:
Sabine Breinig, Lixuan Huang, F. Sima Sariaslani, Grace A. Crum, Arie Ben-Bassat, Todd Vannelli, Nancy W. Rizzo, Amee La Bonte Altenbaugh, Robert J Trotman, Sharon L. Haynie
Publikováno v:
Organic Process Research & Development. 11:278-285
An efficient synthesis of 4-vinylphenol (4-VP) from para-hydroxycinnamic acid (pHCA) that uses the enzyme para-hydroxycinnamic acid decarboxylase (PDC) in a two-phase aqueous−organic solvent has been developed. The 4-VP titer and catalyst productiv
Autor:
Lawrence J. Mersinger, Kelly L. Petrillo, Mark S. Payne, Frederick B. Cooling, John E. Gavagan, Arie Ben-Bassat, Eugenia C. Hann, Shijun Wu, Robert DiCosimo
Publikováno v:
Advanced Synthesis & Catalysis. 347:1125-1131
A thermally-stable nitrile hydratase produced by Comamonas testosteroni 5-MGAM-4D has been expressed in Escherichia coli, and the alginate-immobilized transformant evaluated as catalyst for the conversion of acrylonitrile to acrylamide. In batch reac
Autor:
Eugenia Costa Hann, Kelly L. Petrillo, Mark S. Payne, John E. Gavagan, Frederick B. Cooling, Robert DiCosimo, Arie Ben-Bassat, Shijun Wu
Publikováno v:
Applied Microbiology and Biotechnology. 67:664-670
The genes encoding a thermally stable and regio-selective nitrile hydratase (NHase) and an amidase from Comamonas testosteroni 5-MGAM-4D have been cloned and sequenced, and active NHase has been over-produced in Escherichia coli. Maximal activity req
Publikováno v:
Applied and Environmental Microbiology. 69:5120-5127
The solvent-tolerant strain Pseudomonas putida DOT-T1E has been engineered for biotransformation of toluene into 4-hydroxybenzoate (4-HBA). P. putida DOT-T1E transforms toluene into 3-methylcatechol in a reaction catalyzed by toluene dioxygenase. The
Autor:
Frederick B. Cooling, Amy E. Sigmund, Arie Ben-Bassat, John E. Gavagan, Eugenia C. Hann, Sarita Chauhan, Susan K. Fager, Robert DiCosimo, Mark S. Payne, Susan Marie Hennessey
Publikováno v:
Advanced Synthesis & Catalysis. 345:775-782
Microbial catalysts having a combination of nitrile hydratase and amidase activities had a significantly-higher specific activity for hydrolysis of 3-hydroxyalkanenitriles than microbial nitrilase catalysts. Comamonas testosteroni 22–1, Dietzia sp.
Autor:
Sarita Chauhan, David R. Short, Eugenia Costa Hann, Susan Marie Hennessey, John E. Gavagan, Robert DiCosimo, Amy E. Sigmund, Arie Ben-Bassat, Mark S. Payne, Robert D. Fallon
Publikováno v:
Organic Process Research & Development. 6:492-496
Optimization of microbial cell immobilization, catalyst specific activity, and volumetric productivity were required for scale-up of the nitrilase-catalyzed hydrolysis of 2-methylglutaronitrile to ...
Autor:
R. G. Hall, D. E. Fowler, Beall David S, Arie Ben-Bassat, Joy B. Doran, B. E. Wood, Lonnie O. Ingram
Publikováno v:
Biotechnology Letters. 14:857-862
Hemicellulose and residual starch in corn hulls from wet milling and hemicellulose in corn cobs were hydrolyzed by incubation in dilute sulfuric acid at 140°C to 160°C. These hydrolysates were efficiently fermented to ethanol by a genetically engin