Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing.

Autor: Arias-Arias JL; Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología Universidad de Costa Rica, San José, 11501-2060, Costa Rica.; Dulbecco Lab Studio, Residencial Lisboa 2G, Alajuela, 20102, Costa Rica., Molina-Castro SE; Instituto de Investigaciones en Salud (INISA), Universidad de Costa Rica, San José, 11501-2060, Costa Rica., Monturiol-Gross L; Instituto Clodomiro Picado (ICP), Facultad de Microbiología, Universidad de Costa Rica, San José, 11501-2060, Costa Rica., Lomonte B; Instituto Clodomiro Picado (ICP), Facultad de Microbiología, Universidad de Costa Rica, San José, 11501-2060, Costa Rica., Corrales-Aguilar E; Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología Universidad de Costa Rica, San José, 11501-2060, Costa Rica.

Publikováno v: Biotechnology reports (Amsterdam, Netherlands) [Biotechnol Rep (Amst)] 2023 Mar; Vol. 37, pp. e00780. Date of Electronic Publication: 2022 Dec 31.

MYC dosage compensation is mediated by miRNA-transcription factor interactions in aneuploid cancer.

Autor: Acón M; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; Master Program on Bioinformatics and Systems Biology, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica., Geiß C; Institute for Developmental Biology and Neurobiology, Johannes Gutenberg University, 55128 Mainz, Germany., Torres-Calvo J; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; Master Program on Bioinformatics and Systems Biology, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica., Bravo-Estupiñan D; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; Ph.D. Program in Sciences, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica., Oviedo G; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; Master Program on Bioinformatics and Systems Biology, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica., Arias-Arias JL; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica., Rojas-Matey LA; Master Program on Bioinformatics and Systems Biology, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica., Edwin B; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; Master Program on Bioinformatics and Systems Biology, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica., Vásquez-Vargas G; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica., Oses-Vargas Y; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica., Guevara-Coto J; School of Computer Sciences and Informatics (ECCI), University of Costa Rica, San Jose Costa Rica, 11501-2060 San José, Costa Rica., Segura-Castillo A; Laboratorio de Investigación e Innovación Tecnológica, Universidad Estatal a Distancia (UNED), 474-2050 San José, Costa Rica., Siles-Canales F; Pattern Recognition and Intelligent Systems Laboratory, Department of Electrical Engineering, Universidad de Costa Rica, 11501-2060 San José, Costa Rica.; DC Lab, Lab of Surgery and Cancer, University of Costa Rica, 11501-2060 San José, Costa Rica., Quirós-Barrantes S; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; DC Lab, Lab of Surgery and Cancer, University of Costa Rica, 11501-2060 San José, Costa Rica., Régnier-Vigouroux A; Institute for Developmental Biology and Neurobiology, Johannes Gutenberg University, 55128 Mainz, Germany., Mendes P; Center for Cell Analysis and Modeling and Department of Cell Biology, University of Connecticut School of Medicine, Farmington, 06030 CT, USA., Mora-Rodríguez R; Lab of Tumor Chemosensitivity (LQT), Research Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, 11501-2060 San José, Costa Rica.; Master Program on Bioinformatics and Systems Biology, Postgraduate Program SEP, University of Costa Rica, 11501-2060 San José, Costa Rica.; DC Lab, Lab of Surgery and Cancer, University of Costa Rica, 11501-2060 San José, Costa Rica.; Institute for Developmental Biology and Neurobiology, Johannes Gutenberg University, 55128 Mainz, Germany.

Publikováno v: IScience [iScience] 2021 Nov 08; Vol. 24 (12), pp. 103407. Date of Electronic Publication: 2021 Nov 08 (Print Publication: 2021).

A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging.

Autor: Arias-Arias JL; Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica.; Dulbecco Lab Studio, Residencial Lisboa 2G, Alajuela 20102, Costa Rica., Corrales-Aguilar E; Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica., Mora-Rodríguez RA; Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica.

Publikováno v: Viruses [Viruses] 2021 Jun 22; Vol. 13 (7). Date of Electronic Publication: 2021 Jun 22.

A fluorescence-activatable reporter of flavivirus NS2B-NS3 protease activity enables live imaging of infection in single cells and viral plaques.

Autor: Arias-Arias JL; Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica., MacPherson DJ; Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003., Hill ME; Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003., Hardy JA; Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003., Mora-Rodríguez R; Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José 11501-2060, Costa Rica. Electronic address: rodrigo.morarodriguez@ucr.ac.cr.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2020 Feb 21; Vol. 295 (8), pp. 2212-2226. Date of Electronic Publication: 2020 Jan 09.