Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Ari Lehmusvuori"'
Publikováno v:
BioTechniques, Vol 53, Iss 5, Pp 301-303 (2012)
We have developed a rapid and reliable bacteriophage quantification method based on measurement of phage single-stranded DNA (ssDNA) using switchable lanthanide chelate complementation probes. One oligonucleotide probe contains a non-fluorescent lant
Externí odkaz:
https://doaj.org/article/7fd3aafd76ee46768ae2bf5245e25989
Publikováno v:
IEEE Transactions on Electron Devices. 62:2628-2635
We present a model that can be used to compute the charging and potential at any point of the electrochemical system comprising the ion-sensitive floating gate FET (ISFGFET) exposed to an electrolyte solution. In contrast to ion-sensitive FETs, the s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92898b69f8c2b9da4bc9700e85f6bef0
https://doi.org/10.1109/ted.2015.2441878
https://doi.org/10.1109/ted.2015.2441878
Autor:
Emilia Tuunainen, Minna Soikkeli, P. von Lode, Saara Wittfooth, Ari Lehmusvuori, Titta Seppä, Tero Soukka, Kaisu Rantakokko-Jalava, Ulla Karhunen, Anni Spangar
Publikováno v:
Journal of Microbiological Methods. 118:64-69
Ready to use dry-reagent PCR assays for Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas spp. and for broad-range bacteria detection were developed. The assays were based on novel switchable lanthanide probes that provid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efe8bc7ce3664f433b6ad32f89d1dc63
http://juuli.fi/Record/0006831015
http://juuli.fi/Record/0006831015
Autor:
Ari Lehmusvuori, Matti Kaisti, Anssi Kerko, Tero Soukka, Eero Aarikka, Petri Saviranta, Zhanna A. Boeva
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
Kaisti, M, Kerko, A, Aarikka, E, Saviranta, P, Boeva, Z, Soukka, T & Lehmusvuori, A 2017, ' Real-Time wash-free detection of unlabeled PNA-DNA hybridization using discrete FET sensor ', Scientific Reports, vol. 7, no. 1, 15734, pp. 15734 . https://doi.org/10.1038/s41598-017-16028-7
Scientific Reports
Kaisti, M, Kerko, A, Aarikka, E, Saviranta, P, Boeva, Z, Soukka, T & Lehmusvuori, A 2017, ' Real-Time wash-free detection of unlabeled PNA-DNA hybridization using discrete FET sensor ', Scientific Reports, vol. 7, no. 1, 15734, pp. 15734 . https://doi.org/10.1038/s41598-017-16028-7
Scientific Reports
We demonstrate an electrochemical sensor for detection of unlabeled single-stranded DNA using peptide nucleic acid (PNA) probes coupled to the field-effect transistor (FET) gate. The label-free detection relies on the intrinsic charge of the DNA back
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3abe56b0f7f4a343a4452495e990816
https://doi.org/10.1038/s41598-017-16028-7
https://doi.org/10.1038/s41598-017-16028-7
Autor:
Saara Wittfooth, Minna Soikkeli, Anni Spangar, Titta Seppä, Tero Soukka, Gisela Otto, Klara Petersson, Kaisu Rantakokko-Jalava, Ulla Karhunen, Stefan Scheding, Ari Lehmusvuori, Thomas Laurell, Mikael Evander, Piia von Lode, Pelle Ohlsson, Lisa Mellhammar, Emilia Tuunainen
Publikováno v:
Analytical chemistry. 88(19)
This paper describes an integrated microsystem for rapid separation, enrichment, and detection of bacteria from blood, addressing the unmet clinical need for rapid sepsis diagnostics. The blood sample is first processed in an acoustophoresis chip, wh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc142d05eda78bb55e7b35075a7df5a6
https://pubmed.ncbi.nlm.nih.gov/27264110
https://pubmed.ncbi.nlm.nih.gov/27264110
Publikováno v:
Analytica Chimica Acta. 731:88-92
We introduce a switchable lanthanide luminescence reporter technology based closed-tube PCR for the detection of specific target DNA sequence. In the switchable lanthanide chelate complementation based reporter technology hybridization of two nonfluo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::507664adc6ca0a6bf0712cfde7b6d080
https://doi.org/10.1016/j.aca.2012.04.027
https://doi.org/10.1016/j.aca.2012.04.027
Publikováno v:
BioTechniques. 53:301-303
We have developed a rapid and reliable bacteriophage quantification method based on measurement of phage single-stranded DNA (ssDNA) using switchable lanthanide chelate complementation probes. One oligonucleotide probe contains a non-fluorescent lant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59c37715d933229c0f012fdf22f4bd39
https://doi.org/10.2144/0000113954
https://doi.org/10.2144/0000113954
Publikováno v:
Analytical biochemistry. 465
Genotyping in closed tube is commonly performed using polymerase chain reaction (PCR) amplification and allele-specific oligonucleotide probes using fluorescence resonance energy transfer (FRET). Here we introduce a homogeneous human leukocyte antige
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6d0507bcb068b2145a11a566b591309
https://pubmed.ncbi.nlm.nih.gov/25120130
https://pubmed.ncbi.nlm.nih.gov/25120130
Autor:
Pekka Taimen, Kim Pettersson, Kalle Alanen, Tero Soukka, Riina-Minna Väänänen, Esa Kähkönen, Ari Lehmusvuori, Ulla Karhunen, Saeid Alinezhad
Publikováno v:
Analytical biochemistry. 444
The requirement for high-performance reporter probes in real-time detection of polymerase chain reaction (PCR) has led to the use of time-resolved fluorometry of lanthanide chelates. The aim of this study was to investigate the applicability of the p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d00da5ef05eafc30b7092783aa83756
https://pubmed.ncbi.nlm.nih.gov/24084379
https://pubmed.ncbi.nlm.nih.gov/24084379
Autor:
Jukka Hellman, Ari Lehmusvuori, Alex Azhayev, Tuomas Huovinen, T. Savukoski, Timo Lövgren, Eeva-Christine Brockmann, Urpo Lamminmäki, O. Saavalainen, Sultana Akter, Janne Leivo
Publikováno v:
Protein engineering, designselection : PEDS. 24(9)
Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv antibody library was constructed in single immunoglobulin framework to enable rapid affinity matu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bacce937522408d4ea60c051933ca215
https://pubmed.ncbi.nlm.nih.gov/21680620
https://pubmed.ncbi.nlm.nih.gov/21680620