Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Ari E Friedland"'
Deactivation of the GATA Transcription Factor ELT-2 Is a Major Driver of Normal Aging in C. elegans.
Publikováno v:
PLoS Genetics, Vol 12, Iss 4, p e1005956 (2016)
To understand the molecular processes underlying aging, we screened modENCODE ChIP-seq data to identify transcription factors that bind to age-regulated genes in C. elegans. The most significant hit was the GATA transcription factor encoded by elt-2,
Externí odkaz:
https://doaj.org/article/96798d3d795840328d6a650b02344878
Autor:
Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and devel
Externí odkaz:
https://doaj.org/article/685c8caed9c1480ca04b435e72570dc1
Autor:
Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’, instead of the correct ‘Present address: Department of Genet
Externí odkaz:
https://doaj.org/article/fde8da885a3a48659bda8b8bf4e1a6f3
Autor:
Bei Hopkins, Steven J. Samuelsson, Terence Ta, Haiyan Jiang, Dawn Ciulla, Morgan L. Maeder, Joy E. Horng, Ari E. Friedland, Hoson Chao, Timothy Fennell, Georgia Giannoukos, Maxwell N. Skor, Sebastian Gloskowski, Christine D. Wilson, Rina Mepani, Diana Tabbaa, Gregory Gotta, Vic E. Myer, Jennifer A. DaSilva, Reshica Baral, Eugenio Marco, Alexandra Glucksmann, Deepak Reyon, Vidya Dhanapal, David Bumcrot, Luis A. Barrera, Shivangi Joshi, Tongyao Wang, Michael Stefanidakis, Abhishek Dass, Charles F Albright, Clifford Yudkoff, George S. Bounoutas, Scott Haskett, Shen Shen, Pam Stetkiewicz, Hariharan Jayaram
Publikováno v:
Nature Medicine. 25:229-233
Leber congenital amaurosis type 10 is a severe retinal dystrophy caused by mutations in the CEP290 gene1,2. We developed EDIT-101, a candidate genome-editing therapeutic, to remove the aberrant splice donor created by the IVS26 mutation in the CEP290
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86c0cd9b3d2cffb0a51b7703154f9c31
https://doi.org/10.1038/s41591-018-0327-9
https://doi.org/10.1038/s41591-018-0327-9
Autor:
Luis A. Barrera, Josh Tycko, Patrick D. Hsu, Jonathan S. Gootenberg, Ari E. Friedland, Christine D. Wilson, Xuebing Wu, Vic E. Myer, Omar O. Abudayyeh, Nicholas C. Huston
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
Nature Communications
Nature communications, vol 9, iss 1
Nature Communications
Nature communications, vol 9, iss 1
Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing varia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ada5be5399da0926a9a93e74e6f9b29
https://doaj.org/article/685c8caed9c1480ca04b435e72570dc1
https://doaj.org/article/685c8caed9c1480ca04b435e72570dc1
Autor:
Xuebing Wu, Luis A. Barrera, Ari E. Friedland, Josh Tycko, Vic E. Myer, Jonathan S. Gootenberg, Patrick D. Hsu, Chris Wilson, Omar O. Abudayyeh, Nicholas C. Huston
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
Nature communications, vol 9, iss 1
Nature Communications
Nature communications, vol 9, iss 1
Nature Communications
Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing varia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bd7552eb3bab4b52d3da7ffc9dd05fa
https://doi.org/10.1038/s41467-018-06029-z
https://doi.org/10.1038/s41467-018-06029-z
Autor:
Ari E. Friedland, Monica P. Colaiácovo, George M. Church, Saravanapriah Nadarajan, John A. Calarco, Yonatan B Tzur
Publikováno v:
Genetics. 195:1181-1185
We adapted the CRISPR–Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific ins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::064f9bc3b7cb800a65b6e65151d76678
https://doi.org/10.1534/genetics.113.156075
https://doi.org/10.1534/genetics.113.156075
Autor:
Charlie Albright, Sandra Teixeira, Jamaica Siwak, Abhishek Dass, Scott Haskett, Kiran Gogi, Diana Tabbaa, Eric L. Tillotson, Terence Ta, Fred Harbinski, Emily Brennan, Gregory Gotta, Ramya Viswanathan, Eugenio Marco, Hoson Chao, Frederick Ta, Deepak Reyon, Jen Da Silva, Meltem Isik, Kai-Hsin Chang, Edouard deDreuzy, Haiyan Jiang, Andrew Sadowski, Georgia Giannoukos, Katherine Loveluck, Tongyao Wang, Aditi Chalishazar, John A. Zuris, Abigail Vogelaar, Ari E. Friedland, Jack Heath, Chris Wilson, Minerva E. Sanchez
Publikováno v:
Blood. 132:409-409
Beta hemoglobinopathies resulting from dysfunctional or deficient adult beta-globin expression are some of the most prevalent inherited blood disorders in the world. Upregulation of developmentally-silenced fetal gamma-globin would replace adult beta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6520b0bc5e9bf59489db1d455d8d6922
https://doi.org/10.1182/blood-2018-99-114780
https://doi.org/10.1182/blood-2018-99-114780
Publikováno v:
Science. 324:1199-1202
One, Two, Three Synthetic biologists take much of their inspiration from computing and electrical engineering, attempting to gain programmability in cells using analogous genetic circuits. Friedland et al. (p. 1199 ; see the Perspective by Smolke ) h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::021c9e342513739bb4b176636ea8f4a9
https://doi.org/10.1126/science.1172005
https://doi.org/10.1126/science.1172005
Autor:
John A, Calarco, Ari E, Friedland
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1327
The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4b93b7eb94e40cb14135e68f7b212348
https://pubmed.ncbi.nlm.nih.gov/26423968
https://pubmed.ncbi.nlm.nih.gov/26423968