Development of a thermostable β-glucuronidase-based reporter system for monitoring gene expression in hyperthermophiles

Autor: Aramis A. Villafane, Elisabetta Bini, David Sannino, Maryam Honarbakhsh, Ilona Ruhl

Publikováno v: Biotechnology and Bioengineering. 109:1881-1886

Mesophilic glucuronidases are the most widely used reporters of gene expression in plants, but unsuitable as reporters in (hyper-)thermophiles due their insufficient thermal stability. Here we present the native 66.8 kDa thermostable β-glucuronidase

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83b3d116a9c4480158ed57a72d18e2ab
https://doi.org/10.1002/bit.24432

CopR of Sulfolobus solfataricus represents a novel class of archaeal-specific copper-responsive activators of transcription

Autor: Yekaterina Voskoboynik, Paul H. Blum, Elisabetta Bini, David Sannino, Yukari Maezato, Aramis A. Villafane, Ilona Ruhl

Publikováno v: Microbiology (Reading, England). 157(Pt 10)

In trace amounts, copper is essential for the function of key enzymes in prokaryotes and eukaryotes. Organisms have developed sophisticated mechanisms to control the cytosolic level of the metal, manage its toxicity and survive in copper-rich environ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbe3089e2f95db646059e2d99e34e3a4
https://pubmed.ncbi.nlm.nih.gov/21757491

CopR of Sulfolobus solfataricus represents a novel class of archaeal-specific copper-responsive activators of transcription.

Autor: Villafane A; Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA., Voskoboynik Y; Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA., Ruhl I; Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA., Sannino D; Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA., Maezato Y; Beadle Center for Genetics, University of Nebraska at Lincoln, Lincoln, NE, USA., Blum P; Beadle Center for Genetics, University of Nebraska at Lincoln, Lincoln, NE, USA., Bini E; Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA.

Publikováno v: Microbiology (Reading, England) [Microbiology (Reading)] 2011 Oct; Vol. 157 (Pt 10), pp. 2808-2817. Date of Electronic Publication: 2011 Jul 14.