A prime editor mouse to model a broad spectrum of somatic mutations in vivo.

Autor: Ely ZA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Mathey-Andrews N; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA., Naranjo S; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Gould SI; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Mercer KL; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Newby GA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Cabana CM; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Rideout WM 3rd; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Jaramillo GC; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Harvard-MIT Division of Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, USA., Khirallah JM; Department of Biomedical Engineering, Tufts University, Medford, MA, USA., Holland K; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Angelo State University, San Angelo, TX, USA., Randolph PB; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Freed-Pastor WA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Davis JR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Kulstad Z; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA., Westcott PMK; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Cold Spring Harbor Laboratory, Huntington, NY, USA., Lin L; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Anzalone AV; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Horton BL; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Pattada NB; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Shanahan SL; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Ye Z; Department of Biomedical Engineering, Tufts University, Medford, MA, USA., Spranger S; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Xu Q; Department of Biomedical Engineering, Tufts University, Medford, MA, USA., Sánchez-Rivera FJ; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Jacks T; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA. tjacks@mit.edu.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. tjacks@mit.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2024 Mar; Vol. 42 (3), pp. 424-436. Date of Electronic Publication: 2023 May 11.

Author Correction: Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning.

Autor: Koblan LW; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Arbab M; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Shen MW; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Computational and Systems Biology Program, Massachusetts Institute of Technology, Cambridge, MA, USA., Hussmann JA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Anzalone AV; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Doman JL; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Newby GA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Yang D; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Mok B; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Replogle JM; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA.; Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Xu A; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA.; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA, USA., Sisley TA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Weissman JS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA. weissman@wi.mit.edu.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. weissman@wi.mit.edu., Adamson B; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. badamson@princeton.edu.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. badamson@princeton.edu.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA. badamson@princeton.edu.; Department of Molecular Biology, Princeton University, Princeton, NJ, USA. badamson@princeton.edu., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA. drliu@fas.harvard.edu.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2023 Nov; Vol. 41 (11), pp. 1655.

Author Correction: Engineered pegRNAs improve prime editing efficiency.

Autor: Nelson JW; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Randolph PB; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Shen SP; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Everette KA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Chen PJ; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Anzalone AV; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., An M; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Newby GA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Chen JC; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Hsu A; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA. drliu@fas.harvard.edu.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2022 Mar; Vol. 40 (3), pp. 432.

Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning.

Autor: Koblan LW; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Arbab M; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Shen MW; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Computational and Systems Biology Program, Massachusetts Institute of Technology, Cambridge, MA, USA., Hussmann JA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Anzalone AV; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Doman JL; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Newby GA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Yang D; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Mok B; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Replogle JM; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA.; Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA, USA.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Xu A; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA.; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA, USA., Sisley TA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Weissman JS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, USA. weissman@wi.mit.edu.; Whitehead Institute for Biomedical Research, Cambridge, MA, USA. weissman@wi.mit.edu.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. weissman@wi.mit.edu., Adamson B; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. badamson@princeton.edu.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. badamson@princeton.edu.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA. badamson@princeton.edu.; Department of Molecular Biology, Princeton University, Princeton, NJ, USA. badamson@princeton.edu., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA. drliu@fas.harvard.edu.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2021 Nov; Vol. 39 (11), pp. 1414-1425. Date of Electronic Publication: 2021 Jun 28.

Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.

Autor: Anzalone AV; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Koblan LW; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA. drliu@fas.harvard.edu.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA. drliu@fas.harvard.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2020 Jul; Vol. 38 (7), pp. 824-844. Date of Electronic Publication: 2020 Jun 22.