Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems.

Autor: Prondzynski M; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Berkson P; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Trembley MA; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Tharani Y; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Shani K; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, 02134, USA., Bortolin RH; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Sweat ME; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Mayourian J; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Yucel D; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Cordoves AM; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, 02134, USA., Gabbin B; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Hou C; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA.; Department of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200062, China., Anyanwu NJ; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, 02134, USA., Nawar F; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Cotton J; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Milosh J; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Walker D; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Zhang Y; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Lu F; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Liu X; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA.; Fuwai Hospital, Chinese Academy of Medical Science, Shenzhen, Shenzhen, Guangdong Province, 518057, China., Parker KK; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, 02134, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.; Harvard Stem Cell Institute, Cambridge, MA, 02138, USA., Bezzerides VJ; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA., Pu WT; Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, USA. william.pu@cardio.chboston.org.; Harvard Stem Cell Institute, Cambridge, MA, 02138, USA. william.pu@cardio.chboston.org.

Publikováno v: Nature communications [Nat Commun] 2024 Jul 15; Vol. 15 (1), pp. 5929. Date of Electronic Publication: 2024 Jul 15.