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Autor:
Antony Michael Carr
Publikováno v:
International Journal of Radiation Biology. 66:S133-S139
The response to DNA damaging agents includes a delay to progression through the cell cycle. Irradiation of premitotic cells causes a delay to mitosis and irradiation of G1 and S phase cells causes a delay to DNA synthesis. These delays have become kn
Publikováno v:
Europe PubMed Central
We have developed a DNA-based system, to detect mutations at restriction sites without any selection in culture. DNA is exhaustively digested with a restriction enzyme. Primers flanking a chosen site for this enzyme are used in the polymerase chain r