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pro vyhledávání: '"Antonio Tarragona-Fiol"'
Autor:
Henny J. Eggelte, Stuart Harbron, Brian R. Rabin, Christopher John Taylorson, Antonio Tarragona-Fiol, John M. Ward, E. Sanchez
Publikováno v:
"Protein Engineering, Design and Selection". 6:901-906
In addition to hydrolysing RNA, bovine pancreatic ribonuclease splits esters of pyrimidine nucleoside 3'-phosphates, including dinucleotides. For a series of 3':5'-linked dinucleotides of general structure CpN, where N is a 5' linked nucleoside, kcat
Publikováno v:
Gene. 118:239-245
The coding sequence for the bovine pancreatic ribonuclease (RNase) precursor has been cloned and produced in Escherichia coli using the polymerase chain reaction (PCR) technique. A PCR amplification has been carried out utilizing as template the reco