The transpeptidase PBP2 governs initial localization and activity of the major cell-wall synthesis machinery in E. coli

Autor: Gizem Özbaykal, Eva Wollrab, Francois Simon, Antoine Vigouroux, Baptiste Cordier, Andrey Aristov, Thibault Chaze, Mariette Matondo, Sven van Teeffelen

Publikováno v: eLife, Vol 9 (2020)

Bacterial shape is physically determined by the peptidoglycan cell wall. The cell-wall-synthesis machinery responsible for rod shape in Escherichia coli is the processive 'Rod complex'. Previously, cytoplasmic MreB filaments were thought to govern fo

Externí odkaz: https://doaj.org/article/33683daf65f640baaffd1807dcd757e2

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Autor: Lun Cui, Antoine Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikard

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)

CRISPR interference (CRISPRi) is a method for targeted silencing of transcription that requires the coexpression of protein dCas9 and a customized guide RNA. Here, Cui et al. show that certain guide RNAs induce toxicity in E. coli, and provide design

Externí odkaz: https://doaj.org/article/7ebd4461f9244907aa4954aebfa7e9b5

Computer vision for pattern detection in chromosome contact maps

Autor: Axel Breuer, Axel Cournac, Antoine Vigouroux, Etienne Jean, Adrien Meot, Edgar Oriol, Romain Koszul, Laurent Politis, Arnaud Campeas, Nadège Guiglielmoni, Cyril Matthey-Doret, Lyam Baudry, Vittore F. Scolari, Philippe Henri Chanut, Pierrick Moreau, Rémi Montagne

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.5795. ⟨10.1038/s41467-020-19562-7⟩
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications, 2020, 11 (1), pp.5795. ⟨10.1038/s41467-020-19562-7⟩

Chromosomes of all species studied so far display a variety of higher-order organisational features, such as self-interacting domains or loops. These structures, which are often associated to biological functions, form distinct, visible patterns on g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29132112413e0410fbf43fcd2cafa573
https://doi.org/10.1038/s41467-020-19562-7

Author response: The transpeptidase PBP2 governs initial localization and activity of the major cell-wall synthesis machinery in E. coli

Autor: Andrey Aristov, Baptiste Cordier, Thibault Chaze, Mariette Matondo, Gizem Özbaykal, Antoine Vigouroux, Eva Wollrab, François Simon, Sven van Teeffelen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ae941c8b0fbc615aa73e114856160004
https://doi.org/10.7554/elife.50629.sa2

Class-A penicillin binding proteins do not contribute to cell shape but repair cell- wall defects

Autor: Mariette Matondo, Enno R. Oldewurtel, Sven van Teeffelen, Antoine Vigouroux, Baptiste Cordier, Gizem Özbaykal, Felipe Cava, Andrey Aristov, Laura Alvarez, Thibault Chaze, David Bikard

Publikováno v: eLife
eLife, eLife Sciences Publication, 2020, 9, pp.e51998. ⟨10.7554/eLife.51998⟩
eLife, 2020, 9, pp.e51998. ⟨10.7554/eLife.51998⟩
eLife, Vol 9 (2020)

International audience; Cell shape and cell-envelope integrity of bacteria are determined by the peptidoglycan cell wall. In rod-shaped Escherichia coli, two conserved sets of machinery are essential for cell-wall insertion in the cylindrical part of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ef5d5481e67544cb6615baaf4f56ab4
https://hal.archives-ouvertes.fr/hal-02470461/document