RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit.

Autor: Emilie L Cerezo, Thibault Houles, Oriane Lié, Marie-Kerguelen Sarthou, Charlotte Audoynaud, Geneviève Lavoie, Maral Halladjian, Sylvain Cantaloube, Carine Froment, Odile Burlet-Schiltz, Yves Henry, Philippe P Roux, Anthony K Henras, Yves Romeo

Publikováno v: PLoS Genetics, Vol 17, Iss 6, p e1009583 (2021)

Ribosome biogenesis lies at the nexus of various signaling pathways coordinating protein synthesis with cell growth and proliferation. This process is regulated by well-described transcriptional mechanisms, but a growing body of evidence indicates th

Externí odkaz: https://doaj.org/article/d9e4b8bf8c4a485c8bce6800672ede2b

Genetic analyses led to the discovery of a super-active mutant of the RNA polymerase I.

Autor: Tommy Darrière, Michael Pilsl, Marie-Kerguelen Sarthou, Adrien Chauvier, Titouan Genty, Sylvain Audibert, Christophe Dez, Isabelle Léger-Silvestre, Christophe Normand, Anthony K Henras, Marta Kwapisz, Olga Calvo, Carlos Fernández-Tornero, Herbert Tschochner, Olivier Gadal

Publikováno v: PLoS Genetics, Vol 15, Iss 5, p e1008157 (2019)

Most transcriptional activity of exponentially growing cells is carried out by the RNA Polymerase I (Pol I), which produces a ribosomal RNA (rRNA) precursor. In budding yeast, Pol I is a multimeric enzyme with 14 subunits. Among them, Rpa49 forms wit

Externí odkaz: https://doaj.org/article/c330ec3874884274aab2d5ed7dfd1bc5

The dual life of disordered lysine-rich domains of snoRNPs in rRNA modification and nucleolar compaction

Autor: Carine Dominique, Nana Kadidia Maiga, Alfonso Méndez-Godoy, Benjamin Pillet, Hussein Hamze, Isabelle Léger-Silvestre, Yves Henry, Virginie Marchand, Valdir Gomes Neto, Christophe Dez, Yuri Motorin, Dieter Kressler, Olivier Gadal, Anthony K. Henras, Benjamin Albert

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-19 (2024)

Abstract Intrinsically disordered regions (IDRs) are highly enriched in the nucleolar proteome but their physiological role in ribosome assembly remains poorly understood. Our study reveals the functional plasticity of the extremely abundant lysine-r

Externí odkaz: https://doaj.org/article/3e0b6ba17fdf4c31a03390088eaa8124

Rbp95 binds to 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation

Autor: Priya Bhutada, Sébastien Favre, Mariam Jaafar, Jutta Hafner, Laura Liesinger, Stefan Unterweger, Karin Bischof, Barbara Darnhofer, Devanarayanan Siva Sankar, Gerald Rechberger, Raghida Abou Merhi, Simon Lebaron, Ruth Birner-Gruenberger, Dieter Kressler, Anthony K Henras, Brigitte Pertschy

Publikováno v: Nucleic Acids Research. 50:10053-10077

Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles require

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c308eb0cbc37bcc0b8e82d057bc6129e
https://doi.org/10.1093/nar/gkac724

Making ends meet:a universal driver of large ribosomal subunit biogenesis

Autor: Katherine E. Bohnsack, Anthony K. Henras, Henrik Nielsen, Markus T. Bohnsack

Publikováno v: Bohnsack, K E, Henras, A K, Nielsen, H & Bohnsack, M T 2023, ' Making ends meet : a universal driver of large ribosomal subunit biogenesis ', Trends in Biochemical Sciences, vol. 48, no. 3, pp. 213-215 . https://doi.org/10.1016/j.tibs.2022.09.003

A common aspect of ribosome assembly, conserved across all domains of life, is the establishment of connections between the 5′ and 3′ ends of the large subunit (LSU) ribosomal RNA (rRNA) to initiate rRNA domain compaction and subunit assembly. We

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa09f1df0ef082b7eec0c72a97645d81
https://curis.ku.dk/portal/da/publications/making-ends-meet(77b44864-58fa-4b87-8948-9ce1943a7046).html