Výsledky vyhledávání - "Anthony J. Ashford"

Mitotic chromatin regulates phosphorylation of Stathmin/Op18

Autor: Anthony A. Hyman, Olivier Gavet, Anthony J. Ashford, Eric Karsenti, Søren S. L. Andersen, Andr Sobel, Rgis Tournebize

Publikováno v: Nature. 389:640-643

Meiotic and mitotic spindles are required for the even segregation of duplicated chromosomes to the two daughter cells. The mechanism of spindle assembly is not fully understood, but two have been proposed that are not mutually exclusive1,2,3. The �

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13b3a938463a4c50b25c594853e6bb2a
https://doi.org/10.1038/39382

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Assembly of the 68- and 72-kD Proteins of Signal Recognition Particle with 7S RNA

Autor: Anthony J. Ashford, Michael Remus, Rainer Frank, Bernhard Dobberstein, Henrich Lütcke, Siegfried Prehn

Publikováno v: The Journal of Cell Biology

Signal recognition particle (SRP), the cytoplasmic ribonucleoprotein particle that mediates the targeting of proteins to the ER, consists of a 7S RNA and six different proteins. The 68- (SRP68) and 72- (SRP72) kD proteins of SRP are bound to the 7S R

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e21b651d31eb141e524417022ad4e47a

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Preparation of Tubulin from Porcine Brain

Autor: Anthony J. Ashford, Anthony A. Hyman

Publisher Summary This chapter describes the procedure for preparation of tubulin from porcine brain. Microtubules are polymers of the heterodimeric protein tubulin. Because these heterodimers have the same orientation in the microtubule lattice and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::df92179bac117c9e2d96601465a20244
https://doi.org/10.1016/b978-012164730-8/50094-0

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Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles

Autor: Anthony J. Ashford, Eileen T. O'Toole, Maryse Romao, Thomas Müller-Reichert, Claude Antony, Anthony A. Hyman, Ingrid Sassoon

Publikováno v: Chromosoma. 111(7)

Ndc10p is one of the DNA-binding constituents of the kinetochore in Saccharomyces cerevisiae but light microscopy analysis suggests that Ndc10p is not limited to kinetochore regions. We examined the localization of Ndc10p using immunoelectron microsc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::658c065793176dc4e88d053e67b59190
https://pubmed.ncbi.nlm.nih.gov/12707779

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Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p

Autor: Fedor F. Severin, Paul D. Andrews, Anthony J. Ashford, Ingrid Sassoon, Kenneth B. Kaplan, Anthony A. Hyman, Michael J. R. Stark, Peter K. Sorger, Maria Rita Taba

We have investigated the role of protein phosphorylation in regulation of Saccharomyces cerevisiae kinetochores. By use of phosphatase inhibitors and a type 1 protein phosphatase mutant (glc7-10), we show that the microtubule binding activity, but no

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bc335d213cc4287af5871cb6b391e31
https://europepmc.org/articles/PMC316511/

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Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane-inserted signal sequence

Autor: Bruno Martoglio, Bernhard Dobberstein, Stephen High, Tom A. Rapoport, Angelika Giner, Dirk Görlich, Siegfried Prehn, Anthony J. Ashford, Enno Hartmann, S S Andersen

Publikováno v: The Journal of Biological Chemistry
Europe PubMed Central

A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH2-terminal, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe4c5c1a842fe0f25d99ea2076866af6
https://hdl.handle.net/11858/00-001M-0000-0015-3BE5-311858/00-001M-0000-0015-3BE2-9

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Ultrastructural Localization of Antigens in the Mitotic Spindle of Budding Yeast Using High Presure Freezing and Freeze Substitution

Autor: Thomas Müller-Reichert, Anthony A. Hyman, Claude Antony, Anthony J. Ashford

Publikováno v: Microscopy and Microanalysis. 6:300-301

The preservation of S. cerevisiae ultrastructure is not a trivial task, because the cell wall serves as a diffusion barrier during all steps of preparation. In addition, a high density of proteins in the cytoplasm and in the nucleus renders the visua

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ab73e2e3ca7bf839a9c450123dda6d96
https://doi.org/10.1017/s1431927600033997

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Casein kinase-2 phosphorylates serine-2 in the beta-subunit of initiation factor-2

Autor: Christopher G. Proud, Stephanie J. Clark, Nigel T. Price, Anthony J. Ashford

Publikováno v: Biochimica et biophysica acta. 1010(3)

We have previously presented evidence which suggests that casein kinase-2 phosphorylates a serine residue near the N-terminus of the β-subunit of the initiation factor eIF-2 (Clark, S.J. et al. Biochim. Biophys. Acta 968, 211–219). We now report f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2371c15050c817528a1e43129a9f004
https://pubmed.ncbi.nlm.nih.gov/2493264

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