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pro vyhledávání: '"Anthony Gordon Crewe Hitchcock"'
Autor:
Anna V. Hine, Anthony Gordon Crewe Hitchcock, Richard A. J. Darby, Gareth M. Forde, Siddhartha Ghose, Nigel K. H. Slater
Publikováno v:
Biotechnology and Bioengineering. 95:67-75
Current approaches for purifying plasmids from bacterial production systems exploit the physiochemical properties of nucleic acids in non-specific capture systems. In this study, an affinity system for plasmid DNA (pDNA) purification has been develop
Autor:
Alvin W. Nienow, H. Patel, Julian A. J. Hanak, Anthony Gordon Crewe Hitchcock, Saethawat Chamsart
Publikováno v:
Biotechnology and Bioengineering. 75:387-392
Extensive tests have been carried out to assess the impact of fluid-dynamic-generated stress during alkaline lysis of Escherichia coli cells (host strain DH1 containing the plasmid pTX 0161) to produce a plasmid DNA (pDNA) solution for gene therapy.