Zobrazeno 1 - 10
of 56
pro vyhledávání: '"Anthony G. Sacco"'
Autor:
Renuka Kaul, Satish K. Gupta, Edward C. Yurewicz, Anthony G. Sacco, P. Jethanandani, Chhabi K. Govind
Publikováno v:
Molecular Human Reproduction. 4:1058-1064
Characterization and classification of human zona pellucida glycoproteins is essential to understand the functions of these components during fertilization. To achieve this, antibodies were raised in rabbits against recombinant non-human primate [Bon
Autor:
A Afzalpurka, Anthony G. Sacco, K Chadha, Satish K. Gupta, J D Harris, Siva Kumar Kolluri, Edward C. Yurewicz
Publikováno v:
Biology of Reproduction. 55:410-415
Zona pellucida glycoprotein 3 alpha (ZP3 alpha) has been designated as the primary sperm receptor ligand in porcine gamete interaction. In this study, epitopes were mapped on porcine ZP3 alpha (pZP3 alpha) by using monoclonal antibodies (mAbs) posses
Publikováno v:
Human Reproduction Update. 1:1-18
Contraceptive research has entered a new phase of development with the advent of hybridoma and DNA recombinant technologies. During the past 5 years, significant advances have been made in this area and now it seems that realistic prospects exist for
Publikováno v:
Molecular Reproduction and Development. 36:382-389
The two M(r) 55,000 glycoproteins, ZP3 alpha and ZP3 beta, of porcine zona pellucida copurify as a preparation designated ZP3. Gamete binding assays have implicated ZP3 alpha, but not ZP3 beta, as participating in sperm-zona recognition events. We no
Autor:
Manju Gupta, Harini Bagavant, K. Chadha, Edward C. Yurewicz, Anthony G. Sacco, Satish K. Gupta
Publikováno v:
American Journal of Reproductive Immunology. 30:95-100
PROBLEM Immunization with zona pellucida (ZP) leads to block in fertility with variable degree of ovarian dysfunctions. To design an immunocontraceptive vaccine based on synthetic peptides of zona pellucida, it is imperative to identify and define ep
Autor:
Jeffrey D. Harris, Anthony G. Sacco, Edward C. Yurewicz, Gregory K. Fontenot, David W. Hibler
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1174:211-214
We isolated a cDNA encoding the pig oocyte zona pellucida protein ZP3 alpha from a pig ovary lambda gt11 cDNA library. The 1699 bp cDNA contains a short 3' untranslated region characteristic of cDNAs encoding zona proteins. The deduced amino acid seq
Publikováno v:
Journal of Reproductive Immunology. 23:265-279
Seven monoclonal antibodies (MAs) generated against porcine zona pellucida glycoprotein, ZP3 (comprising both ZP3 alpha and ZP3 beta) were characterized for their specificities to ZP3 alpha (MA 7 and MA 28) or ZP3 beta (MA 1, MA 2, MA 10, MA 27 and M
Publikováno v:
Molecular Reproduction and Development. 33:182-188
The distribution of O-linked oligosaccharides on the M(r) 55,000 glycoproteins, ZP3 alpha and ZP3 beta, of the porcine oocyte zona pellucida was examined. Purified preparations of endo-beta-galactosidase digested ZP3 alpha and ZP3 beta were reduced a
Publikováno v:
Journal of Reproductive Immunology. 20:43-58
Experiments were carried out to study the binding of the major porcine zona pellucida glycoprotein ZP3 (Mr = 55,000) with human FA-1, a fertilization-related antigen isolated from human male germ cell plasma membranes and with GA-1, a germ cell antig
Publikováno v:
American Journal of Primatology. 24:15-28
ZP3 (M, = 55,000) is the major electrophoretic component of the porcine zona pellucida (ZP). In a continuing assessment of ZP3 as a candidate antigen for contraceptive vaccine development, female squirrel monkeys were immunized with 200 μg ZP3 using