Modular design of bi- and multi-specific knob domain fusions

Autor: Mikhail Kuravsky, Glyn F. Gibbons, Callum Joyce, Anthony Scott-Tucker, Alex Macpherson, Alastair D. G. Lawson

Publikováno v: Frontiers in Immunology, Vol 15 (2024)

IntroductionThe therapeutic potential of bispecific antibodies is becoming widely recognised, with over a hundred formats already described. For many applications, enhanced tissue penetration is sought, so bispecifics with low molecular weight may of

Externí odkaz: https://doaj.org/article/7a32fd5f87824e87907bc5f8fdf37044

Identification and characterisation of anti-IL-13 inhibitory single domain antibodies provides new insights into receptor selectivity and attractive opportunities for drug discovery

Autor: Kayleigh Walker, Roberta Baravalle, Rachel Holyfield, Jacqueline Kalms, Helena Wright, Chitra Seewooruthun, Frederick W. Muskett, Anthony Scott-Tucker, Andy Merritt, Alistair Henry, Alastair D. G. Lawson, Gareth Hall, Christine Prosser, Mark D. Carr

Publikováno v: Frontiers in Immunology, Vol 14 (2023)

Interleukin-13 (IL-13) is a cytokine involved in T-cell immune responses and is a well validated therapeutic target for the treatment of asthma, along with other allergic and inflammatory diseases. IL-13 signals through a ternary signalling complex f

Externí odkaz: https://doaj.org/article/ed60b6efb669458b96fb34943581aadf

A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF

Autor: Daniel J. Lightwood, Rebecca J. Munro, John Porter, David McMillan, Bruce Carrington, Alison Turner, Anthony Scott-Tucker, Elizabeth S. Hickford, Antje Schmidt, David Fox, Alison Maloney, Tom Ceska, Tim Bourne, James O’Connell, Alastair D. G. Lawson

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)

TNF can be inhibited by small molecules that stabilize the TNF trimer in an asymmetric conformation. Here, the authors develop a monoclonal antibody that selectively binds this inactive form of TNF, enabling both target engagement assessment and stru

Externí odkaz: https://doaj.org/article/a700e6f6d0e341fa8bbbdf0203d53866

Isolation of antigen-specific, disulphide-rich knob domain peptides from bovine antibodies.

Autor: Alex Macpherson, Anthony Scott-Tucker, Anastasios Spiliotopoulos, Catherine Simpson, Justin Staniforth, Adam Hold, James Snowden, Leah Manning, Jean van den Elsen, Alastair D G Lawson

Publikováno v: PLoS Biology, Vol 18, Iss 9, p e3000821 (2020)

As a novel alternative to established surface display or combinatorial chemistry approaches for the discovery of therapeutic peptides, we present a method for the isolation of small, cysteine-rich domains from bovine antibody ultralong complementarit

Externí odkaz: https://doaj.org/article/aeec4ce75c7e4c63b8811ef6a3660e0a

Exploiting Single Domain Antibodies as Regulatory Parts to Modulate Monoterpenoid Production in E. coli

Autor: Nigel S. Scrutton, Tom Crabbe, Jonathan Wilkes, Anthony Scott-Tucker, Michael John Wright

Publikováno v: Wilkes, J, Scott-tucker, A, Wright, M, Crabbe, T & Scrutton, N 2020, ' Exploiting Single Domain Antibodies as Regulatory Parts to Modulate Monoterpenoid Production in E. coli ', ACS Synthetic Biology, vol. 9, no. 10, pp. 2828-2839 . https://doi.org/10.1021/acssynbio.0c00375

Synthetic biology and metabolic engineering offer potentially green and attractive routes to the production of high value compounds. The provision of high-quality parts and pathways is crucial in enabling the biosynthesis of chemicals using synthetic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::835fe6b4c8fd3a8b8a14ce3df25b379a
https://doi.org/10.1021/acssynbio.0c00375