In vivo generation of human CD19‐CAR T cells results in B‐cell depletion and signs of cytokine release syndrome

Autor: Anett Pfeiffer, Frederic B Thalheimer, Sylvia Hartmann, Annika M Frank, Ruben R Bender, Simon Danisch, Caroline Costa, Winfried S Wels, Ute Modlich, Renata Stripecke, Els Verhoeyen, Christian J Buchholz

Publikováno v: EMBO Molecular Medicine, Vol 10, Iss 11, Pp 1-11 (2018)

Abstract Chimeric antigen receptor (CAR) T cells brought substantial benefit to patients with B‐cell malignancies. Notwithstanding, CAR T‐cell manufacturing requires complex procedures impeding the broad supply chain. Here, we provide evidence th

Externí odkaz: https://doaj.org/article/0d3a5818f8be4f4293451029dc69af29

Surface-Engineered Lentiviral Vectors for Selective Gene Transfer into Subtypes of Lymphocytes

Autor: Annika M. Frank, Christian J. Buchholz

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 12, Iss , Pp 19-31 (2019)

Lymphocytes have always been among the prime targets in gene therapy, even more so since chimeric antigen receptor (CAR) T cells have reached the clinic. However, other gene therapeutic approaches hold great promise as well. The first part of this re

Externí odkaz: https://doaj.org/article/9d7c29c7e9c9498e98ae537f22b45eb4

In Vivo Generation of CAR T Cells Selectively in Human CD4+ Lymphocytes

Autor: Julia D. S. Hanauer, Shiwani Agarwal, Annika M. Frank, Frederic B. Thalheimer, Vanessa Riechert, Christian J. Buchholz

Publikováno v: Molecular Therapy. 28:1783-1794

T cells modified with CD19-specific chimeric antigen receptors (CARs) result in significant clinical benefit for leukemia patients but constitute a challenge for manufacturing. We have recently demonstrated the in vivo generation of CD19-CAR T cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::613bf83411a3dacbf5aa08bea430465f
https://doi.org/10.1016/j.ymthe.2020.05.005

Lentiviral and adeno-associated vectors efficiently transduce mouse T lymphocytes when targeted to murine CD8

Autor: Christian J. Buchholz, Jessica Hartmann, Kathleen Börner, Dorothee M. Günther, Dirk Grimm, Annika M. Frank, Alexander Michels, Mehryad Mataei

Publikováno v: Mol Ther Methods Clin Dev
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss, Pp 334-347 (2021)
Molecular therapy, 23:334-347
Molecular Therapy. Methods & Clinical Development

Preclinical studies on gene delivery into mouse lymphocytes are often hampered by insufficient activity of lentiviral (LV) and adeno-associated vectors (AAVs) as well as missing tools for cell type selectivity when considering in vivo gene therapy. H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd69f9cfa4870088d63bc2fe2cbdb3d5
https://hdl.handle.net/21.11116/0000-000C-795A-821.11116/0000-0009-D149-9

Surface-Engineered Lentiviral Vectors for Selective Gene Transfer into Subtypes of Lymphocytes

Autor: Christian J. Buchholz, Annika M. Frank

Publikováno v: Molecular Therapy. Methods & Clinical Development
Molecular Therapy: Methods & Clinical Development, Vol 12, Iss, Pp 19-31 (2019)

Lymphocytes have always been among the prime targets in gene therapy, even more so since chimeric antigen receptor (CAR) T cells have reached the clinic. However, other gene therapeutic approaches hold great promise as well. The first part of this re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02690d1ec1d62637445af2a3b5f4f4e2
http://europepmc.org/articles/PMC6216101