Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses

Autor: Titli Nargis, Annie R. Piñeros, Sarah C. May, Sarah A. Tersey, Raghavendra G. Mirmira

Publikováno v: STAR Protocols, Vol 4, Iss 1, Pp 101938- (2023)

Summary: Investigating the immune attack on β cells is critical to understanding autoimmune diabetes. Here, we present a protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas, followed by mass cytometric analyses. Thi

Externí odkaz: https://doaj.org/article/7aa06b80963f44238fcbb5c634fb18de

Single-Cell Transcriptional Profiling of Mouse Islets Following Short-Term Obesogenic Dietary Intervention

Autor: Annie R. Piñeros, Hongyu Gao, Wenting Wu, Yunlong Liu, Sarah A. Tersey, Raghavendra G. Mirmira

Publikováno v: Metabolites, Vol 10, Iss 12, p 513 (2020)

Obesity is closely associated with adipose tissue inflammation and insulin resistance. Dysglycemia and type 2 diabetes results when islet β cells fail to maintain appropriate insulin secretion in the face of insulin resistance. To clarify the early

Externí odkaz: https://doaj.org/article/f2e1b6063a2a4a52a958a190d0f6d33f

Proinflammatory signaling in islet β cells propagates invasion of pathogenic immune cells in autoimmune diabetes

Autor: Annie R. Piñeros, Abhishek Kulkarni, Hongyu Gao, Kara S. Orr, Lindsey Glenn, Fei Huang, Yunlong Liu, Maureen Gannon, Farooq Syed, Wenting Wu, Cara M. Anderson, Carmella Evans-Molina, Marcia McDuffie, Jerry L. Nadler, Margaret A. Morris, Raghavendra G. Mirmira, Sarah A. Tersey

Publikováno v: Cell Reports. 39:111011

Type 1 diabetes is a disorder of immune tolerance that leads to death of insulin-producing islet β cells. We hypothesize that inflammatory signaling within β cells promotes progression of autoimmunity within the islet microenvironment. To test this

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5efae739406cad16eb4eff15d0a19e6
https://doi.org/10.1016/j.celrep.2022.111011

Improvement of the resistance against early Mycobacterium tuberculosis-infection in the absence of PI3Kγ enzyme is associated with increase of CD4+IL-17+ cells and neutrophils

Autor: Vania L. D. Bonato, Annie R. Piñeros, M.P. Cavalcanti-Neto, Thaís Barboza Bertolini, Cássia Alves Sérgio, Rafael de Queiroz Prado, Ana Flávia Gembre, Simone G. Ramos

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Given the impossibility to study the lung immune response during Mycobacterium tuberculosis-latent infection, and consequently, the mechanisms that control the bacterial load, it is reasonable to determine the activation of local immunity in the earl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e43b61adc32ed4d2078b9e71906536e0
https://doi.org/10.1016/j.tube.2018.08.009

Sepsis expands a CD39 + plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity

Autor: Paulo Henrique Melo, Luisa Menezes-Silva, Fernando Q. Cunha, Marcos C. Borges, Bernhard Ryffel, Daniel Zoppi, Ícaro Maia Santos de Castro, Juliana E Toller-Kawahisa, Diego B. Caetite, Paula Ramos Viacava, Antonio Edson Rocha Oliveira, Raphael S. Peres, Helder I. Nakaya, Daniele C. Nascimento, Joel Linden, Thiago M. Cunha, Dario S. Zamboni, Gilles Kauffenstein, José C. Alves-Filho, Raphael Gomes Ferreira, Valérie F. J. Quesniaux, Denise Morais da Fonseca, Flávio P. Veras, Paula B. Donate, Annie R. Piñeros, Jonas Augusto Rizzato Paschoal, Marina Alves Damaceno

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Immunity
Immunity, Elsevier, 2021, 54 (9), pp.2024-2041.e8. ⟨10.1016/j.immuni.2021.08.005⟩

Sepsis results in elevated adenosine in circulation. Extracellular adenosine triggers immunosuppressive signaling via the A2a receptor (A2aR). Sepsis survivors develop persistent immunosuppression with increased risk of recurrent infections. We utili

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::478195521c4469e00f8cd9a0d748fe20