Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Annie Aniana"'
Autor:
Annie Aniana, Nashaat T. Nashed, Rodolfo Ghirlando, Leighton Coates, Daniel W. Kneller, Andrey Kovalevsky, John M. Louis
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-14 (2023)
Abstract A critical step for SARS-CoV-2 assembly and maturation involves the autoactivation of the main protease (MProWT) from precursor polyproteins. Upon expression, a model precursor of MProWT mediates its own release at its termini rapidly to yie
Externí odkaz:
https://doaj.org/article/cc30133938ec44e9a71f4b0649ccbb2c
Autor:
Nashaat T. Nashed, Daniel W. Kneller, Leighton Coates, Rodolfo Ghirlando, Annie Aniana, Andrey Kovalevsky, John M. Louis
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-14 (2022)
Structural characterization and catalytic activity of SARS-CoV-2 main protease reveal minimal interface regions enabling dimer formation driven by inhibitor-induced conformational changes of the oxyanion loop.
Externí odkaz:
https://doaj.org/article/1e9e8033fd684980a02f9f29673a1d18
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-9 (2022)
The binding of a drug targeting the active site of a predominantly monomeric SARS-CoV-2 main protease (MProM) favors an equilibrium shift to MProM dimer formation with two equivalent active sites. These results suggest targeting the monomeric active
Externí odkaz:
https://doaj.org/article/613c4d7153af4acfb58e7dadb2dc7ff4
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0160597 (2016)
The transitioning of the ectodomain of gp41 from a pre-hairpin to a six-helix bundle conformation is a crucial aspect of virus-cell fusion. To gain insight into the intermediary steps of the fusion process we have studied the pH and dodecyl phosphoch
Externí odkaz:
https://doaj.org/article/2b9902b48a1649afa656f6d933c16d97
Autor:
John M Louis, Annie Aniana, Katheryn Lohith, Jane M Sayer, Julien Roche, Carole A Bewley, G Marius Clore
Publikováno v:
PLoS ONE, Vol 9, Iss 8, p e104683 (2014)
We previously reported a series of antibodies, in fragment antigen binding domain (Fab) formats, selected from a human non-immune phage library, directed against the internal trimeric coiled-coil of the N-heptad repeat (N-HR) of HIV-1 gp41. Broadly n
Externí odkaz:
https://doaj.org/article/2505e9a47ce943a195d35748e0303366
Publikováno v:
Angewandte Chemie International Edition. 57:5674-5678
Efficient and accurate models to predict the fitness of a sequence would be extremely valuable in protein design. We have explored the use of statistical potentials for the coevolutionary fitness landscape, extracted from known protein sequences, in
Autor:
Robert W. Harrison, Irene T. Weber, Annie Aniana, Joon H. Park, Jane M. Sayer, John M. Louis, Xiaxia Yu
Publikováno v:
Biochemistry. 55(16)
We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) cor
Autor:
Annie Aniana, Irene T. Weber, Chen-Hsiang Shen, Johnson Agniswamy, Jane M. Sayer, John M. Louis
Publikováno v:
Biochemistry. 51:2819-2828
The escape mutant of HIV-1 protease (PR) containing 20 mutations (PR20) undergoes efficient polyprotein processing even in the presence of clinical protease inhibitors (PIs). PR20 shows >3 orders of magnitude decreased affinity for PIs darunavir (DRV
Publikováno v:
Angewandte Chemie International Edition. 57:5953-5953
Publikováno v:
Angewandte Chemie. 130:6061-6061