Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Anni Jacobs"'
Publikováno v:
Molecular Plant-Microbe Interactions, Vol 11, Iss 6, Pp 449-457 (1998)
In view of the recent finding that different T-DNAs tend to ligate and integrate as repeats at single chromosomal positions, the frequency of transformation and cotransformation was determined during cocultivation of Arabidopsis thaliana root explant
Externí odkaz:
https://doaj.org/article/1c9b4f475a794665b313ea903a98c1f1
Autor:
Katleen M.J. Butaye, Inge J.W.M. Goderis, Ann Depicker, Piet F.J. Wouters, Miguel F.C. De Bolle, Stijn L. Delauré, Anni Jacobs, Bruno P. A. Cammue
Publikováno v:
Plant Molecular Biology. 63:533-543
Many studies in both animal and plant systems have shown that matrix attachment regions (MARs) can increase the expression of flanking transgenes. However, our previous studies revealed no effect of the chicken lysozyme MARs (chiMARs) on transgene ex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a49cca9aa60dbbc971fcf0a8c217aff
https://doi.org/10.1007/s11103-006-9107-x
https://doi.org/10.1007/s11103-006-9107-x
Publikováno v:
Molecular Breeding. 9:271-282
To use transgenic potato tubers (Solanum tuberosum cv. Desiree) for bulk production of recombinant antibodies, constructs were engineered for accumulating full-size IgGs and Fab fragments in the plant cell apoplast or endoplasmic reticulum (ER). An i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91f693b9518edc89b43a02f220ffbc82
https://doi.org/10.1023/a:1020306917914
https://doi.org/10.1023/a:1020306917914
Publikováno v:
The Plant Journal. 20:295-304
After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in the plant genome, resulting in the formation of inverted and direct repeats. Because these inverted repeats cannot be amplified and analyz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::461d10367e87190f34bee0d507ad4d8b
https://doi.org/10.1046/j.1365-313x.1999.t01-1-00602.x
https://doi.org/10.1046/j.1365-313x.1999.t01-1-00602.x
Autor:
Anna Depicker, I Strobbe, H. Van Houdt, M. De Neve, M. Van Montagu, Anni Jacobs, S. De Buck, C De Wilde
Publikováno v:
Molecular and General Genetics MGG. 260:582-592
The stability of antibody and Fab expression was assessed in five different homozygous transgenic Arabidopsis lines. Each of these lines showed silencing of the transgenes that encode the antibody polypeptides, leading to instability of antibody prod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45412d564aadb983c03e02bee7f2ab63
https://doi.org/10.1007/s004380050932
https://doi.org/10.1007/s004380050932
Autor:
Chris De Wilde, Geert Angenon, Tom Gerats, Anna Depicker, Geert De Jaeger, Emmanuel S. Buys, Anni Jacobs, Marc Van Montagu, Dominique Eeckhout, Jyoti Kapila
Publikováno v:
European Journal of Biochemistry. 259:426-434
The accumulation of five murine single-chain variable fragments, binding to dihydroflavonol 4-reductase, was analyzed in transgenic Petunia hybrida plants. The five scFv-encoding sequences were cloned in an optimized plant transformation vector for e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb8a9a32d233b46e30be999ac8fd743d
https://doi.org/10.1046/j.1432-1327.1999.00060.x
https://doi.org/10.1046/j.1432-1327.1999.00060.x
Publikováno v:
The Plant Journal. 11:15-29
Nicotiana protoplasts and Arabidopsis leaf discs or roots were co-cultivated with two Agrobacterium strains each carrying a different T-DNA. Co-transformed plants were selected and the integration of the different T-DNAs was analysed at the genetic a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca222eb88e0488e782edfb0f2f064de7
https://doi.org/10.1046/j.1365-313x.1997.11010015.x
https://doi.org/10.1046/j.1365-313x.1997.11010015.x
Publikováno v:
Molecular and General Genetics MGG. 235:389-396
T-DNA vectors were constructed which carry a beta-glucuronidase (gusA) gene fused to the promoter of the nopaline synthase (nos) gene and the 3' end of the octopine synthase (ocs) gene. This reporter gene was cloned at different locations and orienta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d1b0f29b8c5132cee8700884d101a07
https://doi.org/10.1007/bf00279385
https://doi.org/10.1007/bf00279385
Publikováno v:
The Plant journal : for cell and molecular biology. 60(1)
Transgenic loci obtained after Agrobacterium tumefaciens-mediated transformation can be simple, but fairly often they contain multiple T-DNA copies integrated into the plant genome. To understand the origin of complex T-DNA loci, floral-dip and root
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58fc9d36bdae9c9948dca6f12e257056
https://pubmed.ncbi.nlm.nih.gov/19508426
https://pubmed.ncbi.nlm.nih.gov/19508426
Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events
Publikováno v:
Molecular and General Genetics MGG. 224:248-256
During Agrobacterium tumefaciens infection, the T-DNA flanked by 24 bp imperfect direct repeats is transferred and stably integrated into the plant chromosome at random positions. Here we measured the frequency with which a promoterless reporter gene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::405365407be5fd0c029ec4e11600ad36
https://doi.org/10.1007/bf00271558
https://doi.org/10.1007/bf00271558