Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Annette Kamionka"'
Autor:
Miriam Braunstein, Marcus Klotzsche, Mercedes Monteleone, Annette Kamionka, Sabine Ehrt, Wolfgang Hillen, Dirk Schnappinger, Xinzheng V. Guo
Publikováno v:
Journal of Bacteriology. 189:4614-4623
Many processes that are essential for mycobacterial growth are poorly understood. To facilitate genetic analyses of such processes in mycobacteria, we and others have developed regulated expression systems that are repressed by a tetracycline repress
Autor:
Christine Kraft, Ralph Bertram, Wolfgang Hillen, Karin Roth, Marius Majewski, Annette Kamionka
Publikováno v:
Nucleic Acids Research
In this article we report the in vivo and in vitro characterization of single chain tetracycline repressor (scTetR) variants in Escherichia coli. ScTetR is genetically and proteolytically stable and exhibits the same regulatory properties as dimeric
Publikováno v:
Nucleic Acids Research. 32:842-847
We report for the first time the in vitro characterization of a reverse tetracycline repressor (revTetR). The dimeric wild-type repressor (TetR) binds to tet operator tetO in the absence of the inducer anhydrotetracycline (atc) to confer tight repres
Publikováno v:
Journal of Bacteriology. 185:929-937
HPr, the histidine-containing phosphocarrier protein of the bacterial phosphotransferase system (PTS), serves multiple functions in carbohydrate uptake and carbon source regulation in low-G+C-content gram-positive bacteria and in gram-negative bacter
Autor:
Harald Nothaft, Jörg Siepelmeyer, Annette Kamionka, Fritz Titgemeyer, Stephan Parche, Knut Jahreis
Publikováno v:
European Journal of Biochemistry. 269:2143-2150
We have investigated the crr gene of Streptomyces coelicolor that encodes a homologue of enzyme IIAGlucose of Escherichia coli, which, as a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays a key role in carbo
Bacillus subtiliscontains a cyclodextrin-binding protein which is part of a putative ABC-transporter
Autor:
Annette Kamionka, Michael K. Dahl
Publikováno v:
FEMS Microbiology Letters. 204:55-60
Bacillus subtilis is able to grow on alpha-, beta- and gamma-cyclodextrins as a carbon source via a yet unknown metabolizing system. Sequence analysis of the B. subtilis genome reveals that the putative yvfK-yvfO operon seems to be involved in cyclod
Publikováno v:
Antonie van Leeuwenhoek. 78:243-251
Our research group is studying the phosphotransferase system (PTS) of Streptomyces coelicolor, which, in other bacteria, is centrally involved in carbon source uptake and regulation. We have surveyed the public available S. coelicolor genome sequence
Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. We report here the implementation of both approaches with full
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19b5130f81724a1d52325bf9178b3f61
https://europepmc.org/articles/PMC546824/
https://europepmc.org/articles/PMC546824/
Publikováno v:
Journal of bacteriology. 186(13)
We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator ( tetO ) recognition
Autor:
Annette, Kamionka, Stephan, Parche, Harald, Nothaft, Jörg, Siepelmeyer, Knut, Jahreis, Fritz, Titgemeyer
Publikováno v:
European journal of biochemistry. 269(8)
We have investigated the crr gene of Streptomyces coelicolor that encodes a homologue of enzyme IIAGlucose of Escherichia coli, which, as a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays a key role in carbo