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pro vyhledávání: '"Annette J. Goodall"'
Publikováno v:
Biochemistry. 29(48)
The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this re
Autor:
Stephen E. Halford, Annette J. Goodall
Publikováno v:
Biochemistry. 27(5)
The mechanism of action of the EcoRV restriction endonuclease at its single recognition site on the plasmid pAT153 was analyzed by kinetic methods. In reactions at pH 7.5, close to the optimum for this enzyme, both strands of the DNA were cut in a si