Výsledky vyhledávání - "Annelotte M. Vonk"

Akademický článek

High-throughput functional assay in cystic fibrosis patient-derived organoids allows drug repurposing

Autor: Sacha Spelier, Eyleen de Poel, Georgia N. Ithakisiou, Sylvia W.F. Suen, Marne C. Hagemeijer, Danya Muilwijk, Annelotte M. Vonk, Jesse E. Brunsveld, Evelien Kruisselbrink, Cornelis K. van der Ent, Jeffrey M. Beekman

Publikováno v: ERJ Open Research, Vol 9, Iss 1 (2023)

Background Cystic fibrosis (CF) is a rare hereditary disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Recent therapies enable effective restoration of CFTR function of the most common F508del CFTR mu

Externí odkaz: https://doaj.org/article/b2fb8a6f7608451d90c0f47932df4a5b

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Akademický článek

Rationale and design of the HIT-CF organoid study: stratifying cystic fibrosis patients based on intestinal organoid response to different CFTR-modulators

Autor: Peter van Mourik, Sabine Michel, Annelotte M. Vonk, Jeffrey M. Beekman, Cornelis K. van der Ent, on behalf of the HIT-CF consortium

Publikováno v: Translational Medicine Communications, Vol 5, Iss 1, Pp 1-8 (2020)

Abstract Background Cystic fibrosis is a rare recessive monogenic disease caused by loss-of-function mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. Recently developed CFTR-modulators (i.e. drugs that rescue CFTR-fun

Externí odkaz: https://doaj.org/article/97619aed8c814abdb648904ea175743d

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Akademický článek

Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids

Autor: Annelotte M. Vonk, Peter van Mourik, Anabela S. Ramalho, Iris A.L. Silva, Marvin Statia, Evelien Kruisselbrink, Sylvia W.F. Suen, Johanna F. Dekkers, Frank P. Vleggaar, Roderick H.J. Houwen, Jasper Mullenders, Sylvia F. Boj, Robert Vries, Margarida D. Amaral, Kris de Boeck, Cornelis K. van der Ent, Jeffrey M. Beekman

Publikováno v: STAR Protocols, Vol 1, Iss 1, Pp 100019- (2020)

Summary: This protocol describes the isolation, handling, culture of, and experiments with human colon stem cell organoids in the context of cystic fibrosis (CF). In human colon organoids, the function of cystic fibrosis transmembrane conductance reg

Externí odkaz: https://doaj.org/article/fefd7bcad3ec46998e45178ebd7ffc13

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Akademický článek

Rectal Organoids Enable Personalized Treatment of Cystic Fibrosis

Publikováno v: Cell Reports, Vol 26, Iss 7, Pp 1701-1708.e3 (2019)

Summary: In vitro drug tests using patient-derived stem cell cultures offer opportunities to individually select efficacious treatments. Here, we provide a study that demonstrates that in vitro drug responses in rectal organoids from individual patie

Externí odkaz: https://doaj.org/article/dc8d3a11cbb948418c73a2e8f5b11051

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High-throughput functional assay in cystic fibrosis patient-derived organoids allows drug repurposing

Publikováno v: ERJ Open Research. 9:00495-2022

BackgroundCystic fibrosis (CF) is a rare hereditary disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Recent therapies enable effective restoration of CFTR function of the most common F508delCFTRmutat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4461d7310bf86c149acc405022d9288f
https://doi.org/10.1183/23120541.00495-2022

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Validating organoid-derived human intestinal monolayers for personalized therapy in cystic fibrosis

Autor: Liron Birimberg-Schwartz, Wan Ip, Claire Bartlett, Julie Avolio, Annelotte M Vonk, Tarini Gunawardena, Kai Du, Mohsen Esmaeili, Jeffrey M Beekman, Johanna Rommens, Lisa Strug, Christine E Bear, Theo J Moraes, Tanja Gonska

Publikováno v: Life Science Alliance. 6:e202201857

Highly effective drugs modulating the defective protein encoded by the CFTR gene have revolutionized cystic fibrosis (CF) therapy. Preclinical drug-testing on human nasal epithelial (HNE) cell cultures and 3-dimensional human intestinal organoids (3D

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::16ef4274e2edae062d224ddad49590a8
https://doi.org/10.26508/lsa.202201857

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An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay

Autor: Marne C. Hagemeijer, Nikhil T. Awatade, Christian Tischer, Annelotte M. Vonk, Margarida D. Amaral, Jeffrey M. Beekman, Volker Hilsenstein, Iris A.L. Silva, Hugo M. Botelho

Publikováno v: Bioinformatics. 36:5686-5694

MotivationThe forskolin-induced swelling (FIS) assay has become the preferential assay to predict the efficacy of approved and investigational CFTR-modulating drugs for individuals with cystic fibrosis (CF). Currently, no standardized quantification

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66623b3bdb3114a99e8021659d978906
https://doi.org/10.1093/bioinformatics/btaa1073

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High-resolution 3D imaging of fixed and cleared organoids

Autor: Huili Hu, Ellen J. Wehrens, Koen C. Oost, Anne C. Rios, Annelotte M. Vonk, Jane E. Visvader, Hans Clevers, Johanna F. Dekkers, Paul R. Jamieson, Hendrikus C R Ariese, Maria Alieva, Jeffrey M. Beekman, Hugo J. Snippert, Gimano D. Amatngalim, Lianne M Wellens

Publikováno v: Nature Protocols, 14(6), 1756-1771. Nature Publishing Group

In vitro 3D organoid systems have revolutionized the modeling of organ development and diseases in a dish. Fluorescence microscopy has contributed to the characterization of the cellular composition of organoids and demonstrated organoids' phenotypic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e167f6e747cde0439071404446ac4798
https://doi.org/10.1038/s41596-019-0160-8

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