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pro vyhledávání: '"Anne-Catherine Laine"'
Publikováno v:
The Plant Journal. 2:13-24
Summary A laccase-type polyphenoloxidase (EC 1.10.3.2.), abundantly secreted by suspension-cultured sycamore (Acer pseudoplatanus) cells was purified to homogeneity. This laccase form is a glycoprotein (molecular weight 110000) with high mannose and
Publikováno v:
FEBS Letters. 295:179-184
Antibodies specific for xylose-containing plant complex N-linked glycans are used for indirect immunolocalization of xylosyltransferase in sycamore cells. The use of high pressure freezing and freeze substitution for sample preparation resulted in ve
Publikováno v:
Planta. 180:96-104
Suspension-cultured cells of sycamore (Acer pseudoplatanus L.) secrete a number of acid hydrolases and other proteins that have both highmannose and complex asparagine-linked glycans. We used affinity chromatography with concanavalin A and an antiser
Autor:
Loïc Faye, Anne‐Catherine Laine
Publikováno v:
Electrophoresis. 9(12)
Plant glycoproteins generally cross-react because of the presence of identical or related complex glycans which are highly immunogenic. The use of mild periodate oxidation of glycans after glycoprotein transfer from sodium dodecyl sulfate-poly-acryla