Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Anne Tscheließnig"'
Autor:
Anna Christler, Theresa Scharl, Michael Melcher, Johannes Köppl, Anne Tscheließnig, Cabir Toy, Astrid Dürauer, Alois Jungbauer, Dominik G. Sauer
Publikováno v:
Biotechnology and Bioengineering
Technological developments require the transfer to their location of application to make use of them. We describe the transfer of a real‐time monitoring system for lab‐scale preparative chromatography to two new sites where it will be used and de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24c01d3eeb848f75ef6fad5b05663b7c
http://europepmc.org/articles/PMC8518415
http://europepmc.org/articles/PMC8518415
Publikováno v:
Biochemical Engineering Journal. 93:200-211
A protocol for capturing recombinant antibodies was developed with a combination of different precipitation methods. This process comprised four different precipitations, including caprylic acid, polyethylene glycol (PEG), CaCl2, and cold ethanol. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::54bb42bbf5ebb49d2871d22ca085825c
https://doi.org/10.1016/j.bej.2014.10.008
https://doi.org/10.1016/j.bej.2014.10.008
Publikováno v:
Process Biochemistry. 49:2001-2009
A direct capture step was developed for the purification of recombinant antibodies from clarified culture supernatants. This method is a combination of CaCl 2 and polyethylene glycol (PEG) precipitation. For separation of high molecular weight impuri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c613551349ee25a8cb5a14b8a7bf1839
https://doi.org/10.1016/j.procbio.2014.07.012
https://doi.org/10.1016/j.procbio.2014.07.012
Publikováno v:
Biotechnology Journal. 9:766-775
Standard industry processes for recombinant antibody production employ protein A affinity chromatography in combination with other chromatography steps and ultra-/diafiltration. This study compares a generic antibody production process with a recentl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2c3e4a924adfcc8f9589f00c74ca58c
https://doi.org/10.1002/biot.201300480
https://doi.org/10.1002/biot.201300480
Autor:
Alois Jungbauer, Niki S.C. Wong, Ming Wei Wu, Anne Tscheliessnig, Poh Choo Toh, Julita K. Grzeskowiak, Yih Yean Lee, Janet Chusainow
Publikováno v:
ELECTROPHORESIS. 31:1862-1872
For therapeutic antibody production Protein A chromatography is often replaced by non-affinity-based purification sequences, which are considered as more economical. 2-D DIGE was applied for evaluation of scale-up of non-affinity based process of a h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9d254fcb822a0e30ab043bb3b4aa6a3f
https://doi.org/10.1002/elps.200900781
https://doi.org/10.1002/elps.200900781
Autor:
Andre Choo, Jeremy Lee, Gernalia Satianegara, Alois Jungbauer, Anne Tscheliessnig, Danny Ong, Kornelia Schriebl, Siqi Pan
Publikováno v:
Journal of Chromatography A. 1216:7851-7864
A two-step purification strategy comprising of polyethylene glycol (PEG) precipitation and anion-exchange chromatography was developed for a panel of monoclonal immunoglobulin M (IgM) (pI 5.5-7.7) produced from hybridoma cultures. PEG precipitation w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14be94eac424eb0c209fa0103f5316e9
https://doi.org/10.1016/j.chroma.2009.09.059
https://doi.org/10.1016/j.chroma.2009.09.059
Autor:
Yih Yean Lee, Anne Tscheliessnig, Julita K. Grzeskowiak, Janet Chusainow, Poh Choo Toh, Alois Jungbauer, Niki S.C. Wong
Publikováno v:
Protein Expression and Purification. 66:58-65
Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) is an established method for assessing protein expression strategies, understanding pathogenesis mechanisms, characterizing biomarkers, and controlling therapeutic processes. We a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb176188504aed09f24532392f3df9d7
https://doi.org/10.1016/j.pep.2009.01.007
https://doi.org/10.1016/j.pep.2009.01.007
Autor:
Poh Choo Toh, Yih Yean Lee, Niki S.C. Wong, Anne Tscheliessnig, Julita K. Grzeskowiak, Janet Chusainow, Alois Jungbauer
Publikováno v:
Journal of Chromatography A. 1216:4902-4912
Although Staphylococcus Protein A (SpA) affinity chromatography is the state of the art capture step for antibody purification, non-affinity methods are more economical. We used two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d0ad49d5660910fa96821f8afc6876e
https://doi.org/10.1016/j.chroma.2009.04.014
https://doi.org/10.1016/j.chroma.2009.04.014
Autor:
Alois Jungbauer, Anne Tscheliessnig
Publikováno v:
Journal of Chromatography A. 1216:2676-2682
High-performance monolith affinity chromatography employing protein A resins has been introduced previously for the fast purification of IgG from different sources. Here we describe the design and evaluation of a fast and specific method for quantita
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a1fcb8234622472b12a90e9ca1734d8
https://doi.org/10.1016/j.chroma.2008.07.063
https://doi.org/10.1016/j.chroma.2008.07.063
Autor:
Allen Chen, Yih Yean Lee, Jeremy Lee, Gloria Adduci, Andre Choo, Alois Jungbauer, Anne Tscheliessnig
Publikováno v:
Journal of Chromatography A. 1216:2683-2688
An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were signi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae71248dc41b1818c8040d4d8400b6d7
https://doi.org/10.1016/j.chroma.2008.10.067
https://doi.org/10.1016/j.chroma.2008.10.067