Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Anne Stern"'
Autor:
Adelbert Grossmann, Hauke Lilie, Rainer Rudolph, Elisabeth Schwarz, Anke Rattenholl, Anne Stern
Publikováno v:
European Journal of Biochemistry. 268:3296-3303
Nerve growth factor (beta-NGF), a neurotrophin required for the development and survival of specific neuronal populations, is translated as a prepro-protein in vivo. While the presequence mediates translocation into the endoplasmic reticulum, the fun
Autor:
Ulrich Kohnert, Rainer Rudolph, Jan H. Verheijen, E. Jacoline, D. Weening-Verhoeff, Anne Stern, Ulrich Opitz, Ulrich Martin, Helmut Lill, Heinrich Prinz, Max Lechner, Georg-B. Kresse, Peter Bucket, Stephan Fischer
Publikováno v:
"Protein Engineering, Design and Selection". 5:93-100
BM 06.022 is a t-PA deletion variant which comprises the kringle 2 and the protease domain. Production of BM 06.022 in Escherichia coli leads to the formation of inactive inclusion bodies, which have to be refolded by an in vitro refolding process to
Autor:
Anne Stern, Karin Wolff
Publikováno v:
FEMS Microbiology Letters. 83:179-185
The class 3 protein (PorB) is an important component of the meningococcal outer membrane. The structural gene (porB) encoding the class 3 protein has been cloned using primers suitable for the amplification of the corresponding chromosomal fragment b
Autor:
Ulrich H. Weidle, Anne Stern
Publikováno v:
Gene. 87:305-308
The functions of the finger and kringle-2 (K2) domains of human tissue-type plasminogen activator (t-PA) in mediating fibrin-stimulated plasminogenolytic activity are well documented. Contradictory results have been reported for the kringle-1 (K1) do
Publikováno v:
Thrombosis and haemostasis. 76(6)
SummaryRecent in vitro studies have shown that although recombinant Escherichia coli-produced protease domain of tissue-type plasminogen activator (t-PA) has no appreciable fibrin binding and less plasmin-forming activity compared to the wild-type, i
Publikováno v:
Nature biotechnology. 14(4)
Erythrina trypsin/tPA inhibitor (ETI) from the seeds of Erythrina caffra retains its native structure and inhibitory function after reducing its two disulfide bonds. In order to elucidate the specific role of these crosslinks, alanine residues were s
Autor:
Anne Stern, Karin Wolff
Publikováno v:
FEMS microbiology letters. 125(2-3)
The distribution of distinct sequences in pathogenic and commensal Neisseria species was investigated systematically by dot blot analysis. Probes representing the genes of Rmp, pilin and IgA1 protease were found to hybridize exclusively to the chromo
Publikováno v:
Nucleic acids research. 20(17)
Autor:
Thomas F. Meyer, Anne Stern, K. S. Bhat, S. G. Morrison, Eva-Maria Kupsch, John Swanson, O. Barrera, Carol P. Gibbs, Fritz Jähnig
Publikováno v:
Molecular microbiology. 5(8)
Summary Variants of Neisseria gonorrhoeae MS11 show distinct colony morphologies because of the expression of a class of surface components called opacity (Opa, PII) proteins. Southern analyses combined with molecular cloning of genomic DNA from a si
Autor:
Stephan Fischer, Klaus Strein, Ulrich Kohnert, Ulrich Opitz, Anne Stern, Gisbert Sponer, Rainer Rudolph, Ulrich Martin
Publikováno v:
Thrombosis and haemostasis. 65(5)
SummaryThe recombinant plasminogen activator BM 06.022 consists of the kringle 2 and the protease domains of human t-PA and is unglycosylated because of the expression in Escherichia coli The thrombolytic and pharmacokinetic properties as well as the