A versatile system for USER cloning-based assembly of expression vectors for mammalian cell engineering.

Autor: Anne Mathilde Lund, Helene Faustrup Kildegaard, Maja Borup Kjær Petersen, Julie Rank, Bjarne Gram Hansen, Mikael Rørdam Andersen, Uffe Hasbro Mortensen

Publikováno v: PLoS ONE, Vol 9, Iss 5, p e96693 (2014)

A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammali

Externí odkaz: https://doaj.org/article/181d730b6705404b876e3f3957db1fb4

Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Autor: Julian Brandl, Mikael Rørdam Andersen, Helene Faustrup Kildegaard, Claus Kristensen, Anne Mathilde Lund, Lasse Ebdrup Pedersen, Christian Schrøder Kaas

Publikováno v: BMC Systems Biology
Lund, A M, Kaas, C S, Brandl, J, Pedersen, L E, Kildegaard, H F, Kristensen, C & Andersen, M R 2017, ' Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data ', B M C Systems Biology, vol. 11, no. 1, 37 . https://doi.org/10.1186/s12918-017-0414-4
Lund, A M, Kaas, C S, Brandl, J, Pedersen, L E, Kildegaard, H F, Kristensen, C & Andersen, M R 2017, ' Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data ', BMC Systems Biology, vol. 11, no. 1, 37 . https://doi.org/10.1186/s12918-017-0414-4

Background Protein secretion is one of the most important processes in eukaryotes. It is based on a highly complex machinery involving numerous proteins in several cellular compartments. The elucidation of the cell biology of the secretory machinery

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94006e7c43ddde148668ebe5d0603d29
https://doi.org/10.1186/s12918-017-0414-4

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

Autor: Stefan Kol, Johan Rockberg, Gyun Min Lee, Claes Nymand Nilsson, Magnus Lundqvist, Mikael Rørdam Andersen, Helene Faustrup Kildegaard, Anne Mathilde Lund, Henning Gram Hansen, Lise Marie Grav

Publikováno v: Hansen, H G, Nilsson, C N, Lund, A M, Kol, S, Grav, L M, Lundqvist, M, Rockberg, J, Min Lee, G, Andersen, M R & Kildegaard, H F 2015, ' Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells ', Scientific Reports, vol. 5, 18016 . https://doi.org/10.1038/srep18016
Scientific Reports

Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of n

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::736bc41ebe39efca30fe3c195d386c5c
https://orbit.dtu.dk/en/publications/41719bc3-f624-40be-ba5e-4449c22aac7f

RNA-seq based expression analysis of the CHO cell protein secretion pathway

Autor: Anne Mathilde Lund, Christian Schrøder Kaas, Helene Faustrup Kildegaard, Claus Kristensen, Mikael Rørdam Andersen

Publikováno v: Lund, A M, Kaas, C S, Kildegaard, H F, Kristensen, C & Andersen, M R 2013, ' RNA-seq based expression analysis of the CHO cell protein secretion pathway ' 3rd Annual Next Generation Sequencing Asia Congress, Singapore, Singapore, 08/10/2013-09/10/2013, .
Technical University of Denmark Orbit

The Chinese hamster ovary (CHO) cell-line is the predominant mammalian industrial cell line being used to produce recombinant therapeutic proteins. Although CHO cells have been used for more than 25 years, the genome sequence was first published in 2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::da0cef51381b435992c3fdfa3ce3a147
http://orbit.dtu.dk/en/publications/rnaseq-based-expression-analysis-of-the-cho-cell-protein-secretion-pathway(11526c30-1839-4e41-9edf-dfc09c0e9eeb).html

A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering

Autor: Mikael Rørdam Andersen, Anne Mathilde Lund, Uffe Hasbro Mortensen, Helene Faustrup Kildegaard, Maja Borup Kjær Petersen, Bjarne Gram Hansen, Julie Rank

Publikováno v: PLoS ONE, Vol 9, Iss 5, p e96693 (2014)
PLoS ONE
Lund, A M, Kildegaard, H F, Petersen, M B K, Rank, J, Hansen, B G, Andersen, M R & Mortensen, U H 2014, ' A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering ', P L o S One, vol. 9, no. 5, e96693 . https://doi.org/10.1371/journal.pone.0096693

A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique – USER cloning – to rapidly construct m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ec1c5ed8da48a59ffdbfcc1f6d1b25b
https://doi.org/10.1371/journal.pone.0096693