Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation

Autor: David E. Cohn, Stephanie C. Weber, Baljyot Parmar, Samantha Graydon Tope, Albright Kim, Rodrigo Reyes-Lamothe, Nicolas Soubry, Stefan Biedzinski, James Wall, Anne-Marie Ladouceur

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance Bacterial cells are small and were long thought to have little to no internal structure. However, advances in microscopy have revealed that bacteria do indeed contain subcellular compartments. But how these compartments form has remained

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93ee1bac0b9403402239312388b26ee5
http://europepmc.org/articles/PMC7414142

Fluorescence Microscopy Light Source Review

Autor: Anne-Marie Ladouceur, Claire M. Brown

Publikováno v: Current Protocols. 1

Traditional arc-based light sources and Light Emitting Diodes (LEDs) are described, and the pros and cons of these sources with respect to fluorescence microscopy are discussed. For multi-color applications, arc-based light sources offer white light

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3553abb784fe27ba0e286f7013c6868d
https://doi.org/10.1002/cpz1.243

Microtubule Dynamics Scale with Cell Size to Set Spindle Length and Assembly Timing

Autor: Nicolas Minc, Gaëlle Letort, Laras Pitayu, Julie C. Canman, Benjamin Lacroix, Anne Marie Ladouceur, Marine Stefanutti, Paul S. Maddox, Amy Shaub Maddox, François Nédélec, Julien Dumont, Gilliane Maton, Jérémy Sallé

Publikováno v: Developmental Cell
Developmental Cell, Elsevier, 2018, 45 (4), pp.496-511.e6. ⟨10.1016/j.devcel.2018.04.022⟩
Developmental Cell, 2018, 45 (4), pp.496-511.e6. ⟨10.1016/j.devcel.2018.04.022⟩

International audience; Successive cell divisions during embryonic cleavage create increasingly smaller cells, so intracellular structures must adapt accordingly. Mitotic spindle size correlates with cell size, but the mechanisms for this scaling rem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f79c32facb0aef9463e66f526f4d83fc
https://www.repository.cam.ac.uk/handle/1810/316718

Cdk1-dependent regulation of the Mre11 complex couples DNA repair pathways to cell cycle progression

Autor: Anne-Marie Ladouceur, Antoine Simoneau, Damien D’Amours, Xavier Robellet

Publikováno v: Cell Cycle. 13:1078-1090

Homologous recombination (HR) and non-homologous end joining (NHEJ) are the main pathways ensuring the repair of DNA double-stranded breaks (DSBs) in eukaryotes. It has long been known that cell cycle stage is a major determinant of the type of pathw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b42c054baa0c5891a289804ae8f2f0c9
https://doi.org/10.4161/cc.27946

Octameric CENP-A Nucleosomes Are Present at Human Centromeres throughout the Cell Cycle

Autor: Jonas F. Dorn, Abbas Padeganeh, J. Boisvert, Joel Ryan, Paul S. Maddox, Anne Marie Ladouceur

Publikováno v: Current Biology. 23(9):764-769

SummaryThe presence of a single centromere on each chromosome that signals formation of a mitotic kinetochore is central to accurate chromosome segregation [1]. The histone H3 variant centromere protein-A (CENP-A) is critical for centromere identity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a6b3f9b866bb39bc7fd1a8a88cd7b93