Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Anne M. van der Valk"'
Autor:
Anne M. van der Valk, Ariel Louwrier
Publikováno v:
Enzyme and Microbial Technology. 36:947-952
In the past, Taq polymerase was reversibly inactivated by modification with a dicarboxylic acid anhydride in aqueous media, to enable ‘hot start PCR’. However, there are various constraints in using such a method including temperature and concent
Autor:
Meg C. O'Shaughnessy, Simon C. Baker, David N. Howbrook, Anne M. van der Valk, Dipak K. Sarker, Andrew W. Lloyd
Publikováno v:
Drug Discovery Today. 8:642-651
The focus of high-throughput drug discovery has progressed through the genome and the transcriptome and is now moving towards more difficult problems in assessing the proteome, glycome and metabolome. Microarrays are currently the major tool in the a
Autor:
David N. Howbrook, Ariel Louwrier, Simon C. Baker, Anne M. van der Valk, Andrew W. Lloyd, Meg C. O'Shaughnessy, Dipak K. Sarker
Publikováno v:
Biotechnology Letters. 25:1325-1328
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biot
Autor:
Ariel Louwrier, Anne M. van der Valk
Publikováno v:
Biotechnology Letters. 23:175-178
Sucrose or trehalose at 0.3 M stabilised double-stranded DNA in PCR. Furthermore, the DNA polymerase from Thermus aquaticus was stabilised towards thermo-denaturation at 98 °C by sucrose or trehalose. The effect was limited to these sugars and was s
Autor:
Anne M. van der Valk
Publikováno v:
Drug Discovery Today. 8:781-783
Autor:
Anne M, van der Valk, David N, Howbrook, Meg C, O'Shaughnessy, Dipak K, Sarker, Simon C, Baker, Ariel, Louwrier, Andrew W, Lloyd
Publikováno v:
Biotechnology letters. 25(16)
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biot