The checkpoint protein Zw10 connects CAL1-dependent CENP-A centromeric loading and mitosis duration in Drosophila cells.

Autor: Anne-Laure Pauleau, Andrea Bergner, Janko Kajtez, Sylvia Erhardt

Publikováno v: PLoS Genetics, Vol 15, Iss 9, p e1008380 (2019)

A defining feature of centromeres is the presence of the histone H3 variant CENP-A that replaces H3 in a subset of centromeric nucleosomes. In Drosophila cultured cells CENP-A deposition at centromeres takes place during the metaphase stage of the ce

Externí odkaz: https://doaj.org/article/1d011ab7f47b4053b2c93848c59865c7

TIAR marks nuclear G2/M transition granules and restricts CDK 1 activity under replication stress

Autor: Andrés J. López-Contreras, Marius Bruer, Katharina Haneke, Sylvia Erhardt, Lea Roessig, Hsu Min Sung, Georg Stoecklin, Anne Laure Pauleau, Vanesa Lafarga, Sara Rodriguez-Acebes, Juan Méndez, Oscar Fernandez-Capetillo, Oliver J. Gruss

Publikováno v: Lafarga, V, Sung, H-M, Haneke, K, Roessig, L, Pauleau, A-L, Bruer, M, Rodriguez-Acebes, S, Lopez-Contreras, A J, Gruss, O J, Erhardt, S, Mendez, J, Fernandez-Capetillo, O & Stoecklin, G 2019, ' TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress ', EMBO Reports, vol. 20, no. 1, e46224 . https://doi.org/10.15252/embr.201846224
Repisalud
Instituto de Salud Carlos III (ISCIII)

The G2/M checkpoint coordinates DNA replication with mitosis and thereby prevents chromosome segregation in the presence of unreplicated or damaged DNA Here, we show that the RNA-binding protein TIAR is essential for the G2/M checkpoint and that TIAR

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ce61c87182cf6208a4ea8463b1016bc
https://doi.org/10.15252/embr.201846224

The E3 Ligase CUL3/RDX Controls Centromere Maintenance by Ubiquitylating and Stabilizing CENP-A in a CAL1-Dependent Manner

Autor: Anne-Laure Pauleau, Debora Bade, Sylvia Erhardt, Astrid Wendler

Publikováno v: Developmental Cell. 28:508-519

SummaryCentromeres are defined by the presence of the histone H3 variant CENP-A in a subset of centromeric nucleosomes. CENP-A deposition to centromeres depends on a specialized loading factor from yeast to humans that is called CAL1 in Drosophila. H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4db5752069063f9f0650daefa6abc11
https://doi.org/10.1016/j.devcel.2014.01.031

Real-time imaging of the intracellular glutathione redox potential

Autor: Thorsten Brach, Guido H. Wabnitz, Tobias P. Dick, Anne Laure Pauleau, Yvonne Samstag, Laurent Marty, Marcus Gutscher, Andreas J. Meyer

Publikováno v: Nature Methods. 5:553-559

Dynamic analysis of redox-based processes in living cells is now restricted by the lack of appropriate redox biosensors. Conventional redox-sensitive GFPs (roGFPs) are limited by undefined specificity and slow response to changes in redox potential.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff674b450b40c6342e1eb46920a202c5
https://doi.org/10.1038/nmeth.1212

The apoptosis/autophagy paradox: autophagic vacuolization before apoptotic death

Autor: Eeva-Liisa Eskelinen, Paul Saftig, Abdelali Jalil, Anne-Laure Pauleau, Gérard Pierron, Rosa-Ana Gonzalez-Polo, Nathanael Larochette, Guido Kroemer, Sylvie Souquère, Patricia Boya

Publikováno v: Journal of Cell Science. 118:3091-3102

Autophagic cell death is morphologically characterized by an accumulation of autophagic vacuoles. Here, we show that inactivation of LAMP2 by RNA interference or by homologous recombination leads to autophagic vacuolization in nutrient-depleted cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4685bcd0c1079153542696c4ecbc1cb
https://doi.org/10.1242/jcs.02447