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A hexanucleotide selected for increased cellular uptake in cis contains a highly active CpG-motif in human B cells and primary peripheral blood mononuclear cells

Autor: Miriam Mende, Anke Detzer, Marita Overhoff, Anne Hopert, Georg Sczakiel, Holger Kirchner, Kirsten Börngen, Peter Schlenke, Winfried Wünsche

Publikováno v: Immunology. 120:261-272

Summary The relationship between immunostimulation of human B cells by cytosine–phosphate–guanosine (CpG) -containing oligonucleotides and their physical cellular uptake is of mechanistic interest and a prerequisite for rational improvements of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ce238920a4abfb45eb927ba21d9c1ab
https://doi.org/10.1111/j.1365-2567.2006.02497.x

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Analysis of Plasmacytoid and Myeloid Dendritic Cells in Nasal Epithelium

Autor: Brigitte Mack, Gunther Hartmann, Evelyn Hartmann, Hendrik Graefe, Stefan Endres, Ralph Pries, Anne Hopert, Simon Rothenfusser, Barbara Wollenberg, Hendrik Poeck

Publikováno v: Clinical and Vaccine Immunology. 13:1278-1286

The role of plasmacytoid dendritic cells (PDC), the major producers of alpha interferon upon viral infection, in the nasal mucosa is largely unknown. Here we examined the presence of PDC together with myeloid dendritic cells (MDC) in the nasal epithe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0548587105eccebcc4840c79eb280e04
https://doi.org/10.1128/cvi.00172-06

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Treatment of mycoplasma contamination in a large panel of cell cultures

Autor: Cord C. Uphoff, Suzanne M. Gignac, Zhen-Bo Hu, Maren Voges, Elke Fleckenstein, Anne Hopert, Hans G. Drexler

Publikováno v: In Vitro Cellular & Developmental Biology - Animal. 30:344-347

Mycoplasmal contamination remains a significant impediment to the culture of eukaryotic cells. For certain cultures, attempts to eliminate the infection are feasible alternatives to the normally recommended disposal of the contaminated culture. Here,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e62d0b81561c88290aedf3e546159051
https://doi.org/10.1007/bf02631456

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Mycoplasma detection by PCR analysis

Autor: Hans G. Drexler, Manfred Wirth, Anne Hopert, Cord C. Uphoff, Hansjörg Hauser

Publikováno v: In Vitro Cellular & Developmental Biology - Animal. 29:819-821

The polymerase chain reaction (PCR) method was used to detect mycoplasma contamination in a panel of 42 continuous cell lines. According to the microbiological cultivation assay on agar, 29 cell lines were chronically infected and 13 cell lines were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7dd5281cad6e47b8f303ce59572f6ef6
https://doi.org/10.1007/bf02634350

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Specificity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell lines

Autor: Manfred Wirth, Hans G. Drexler, Anne Hopert, Hansjörg Hauser, Cord C. Uphoff

Publikováno v: Journal of immunological methods. 164(1)

The polymerase chain reaction (PCR) amplification was used for the detection of mycoplasma contamination in 42 continuous cell lines. Using the microbiological cultivation on agar as the reference method, 29 cell lines were regarded as positive and 1

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbe1e6b86418b98f480178b75ef4bc5e
https://pubmed.ncbi.nlm.nih.gov/8360512

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