Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins:A case study of a bacterially expressed recombinant biopharmaceutical protein

Autor: Marie Grimstrup, Ejvind Mørtz, Jakob Bunkenborg, Peter Højrup, Anne Fich Holmbjerg, Max Per Kristiansen, Thomas Kofoed, Søren Heissel

Publikováno v: Heissel, S, Bunkenborg, J, Kristiansen, M P, Holmbjerg, A F, Grimstrup, M, Mørtz, E, Kofoed, T & Højrup, P 2018, ' Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins : A case study of a bacterially expressed recombinant biopharmaceutical protein ', Protein Expression and Purification, vol. 147, pp. 69-77 . https://doi.org/10.1016/j.pep.2018.03.002

Recombinantly expressed biopharmaceutical proteins often undergo a series of purification steps with the aim of removing contaminating material. Depending on the application of the protein, there are various requirements for the degree of purity, but

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