CRISPRi screen for enhancing heterologous α-amylase yield in Bacillus subtilis

Autor: Adrian Sven Geissler, Annaleigh Ohrt Fehler, Line Dahl Poulsen, Enrique González-Tortuero, Thomas Beuchert Kallehauge, Ferhat Alkan, Christian Anthon, Stefan Ernst Seemann, Michael Dolberg Rasmussen, Anne Breüner, Carsten Hjort, Jeppe Vinther, Jan Gorodkin

Publikováno v: Geissler, A S, Fehler, A O, Poulsen, L D, Gonzalez-Tortuero, E, Kallehauge, T B, Alkan, F, Anthon, C, Seemann, S E, Rasmussen, M D, Breuener, A, Hjort, C, Vinther, J & Gorodkin, J 2023, ' CRISPRi screen for enhancing heterologous alpha-amylase yield in Bacillus subtilis ', Journal of Industrial Microbiology and Biotechnology, vol. 50, no. 1, kuac028 . https://doi.org/10.1093/jimb/kuac028

Yield improvements in cell factories can potentially be obtained by fine-tuning the regulatory mechanisms for gene candidates. In pursuit of such candidates, we performed RNA-sequencing of two α-amylase producing Bacillus strains and predict hundred

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8ab4bc4d4013dd5d0ff231de64602d9
https://doi.org/10.1093/jimb/kuac028

BSGatlas: a unified

Autor: Adrian Sven, Geissler, Christian, Anthon, Ferhat, Alkan, Enrique, González-Tortuero, Line Dahl, Poulsen, Thomas Beuchert, Kallehauge, Anne, Breüner, Stefan Ernst, Seemann, Jeppe, Vinther, Jan, Gorodkin

Publikováno v: Microbial Genomics

A large part of our current understanding of gene regulation in Gram-positive bacteria is based on Bacillus subtilis , as it is one of the most well studied bacterial model systems. The rapid growth in data concerning its molecular and genomic biolog

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::98caddc191271fc7b28d6c2e53c4ea53
https://pubmed.ncbi.nlm.nih.gov/33539279

Ribosomal dimerization factor YfiA is the major protein synthesized after abrupt glucose depletion in Lactococcus lactis

Autor: Karin Hammer, Dorte Frees, Pekka Varmanen, Jan Martinussen, Anne Breüner, Anna Monika Boguta, Mogens Kilstrup

Publikováno v: Breuner, A, Frees, D, Varmanen, P, Boguta, A M, Hammer, K, Martinussen, J & Kilstrup, M 2016, ' Ribosomal dimerization factor YfiA is the major protein synthesized after abrupt glucose depletion in Lactococcus lactis ', Microbiology (United Kingdom), vol. 162, pp. 1829-1839 . https://doi.org/10.1099/mic.0.000362

We analysed the response of the model bacterium Lactococcus lactis to abrupt depletion of glucose after several generations of exponential growth. Glucose depletion resulted in a drastic drop in the energy charge accompanied by an extremely low GTP l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a7f80991ac6dedb5c79c554f7cd51aa
https://pubmed.ncbi.nlm.nih.gov/27557864

Resolvase-like recombination performed by the TP901-1 integrase The GenBank accession number for the sequence reported in this paper is Y15043

Autor: Karin Hammer, Anne Breüner, Lone Brøndsted

Publikováno v: Microbiology. 147:2051-2063

The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 is unusual in several respects. First, the integrase belongs to the family of extended resolvases rather than to the λ integrase family and second, in the presence

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::efa54a15c48ff444088b7bc12f50f9ab
https://doi.org/10.1099/00221287-147-8-2051

The centromere‐like parC locus of plasmid R1

Autor: Mette Dam, Anne Breüner, Susanne Møller Pedersen, Rasmus Bugge Jensen, Kenn Gerdes

Publikováno v: Molecular Microbiology. 20:581-592

The parA partitioning system of plasmid R1 consists of three components: the cis-acting centromere-like parC locus, and two proteins, ParM and ParR. The parC locus contains two sets of five direct repeats (iterons) to which the ParR protein binds. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0eb1e538eaf23e16dfeacdf4e24a417c
https://doi.org/10.1046/j.1365-2958.1996.5351063.x