Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Autor: Pernille von Freiesleben, Nikolaj Spodsberg, Anne Stenbæk, Henrik Stålbrand, Kristian B. R. M. Krogh, Anne S. Meyer

Publikováno v: Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-14 (2018)

Abstract Background Softwood is a promising feedstock for lignocellulosic biorefineries, but as it contains galactoglucomannan efficient mannan-degrading enzymes are required to unlock its full potential. Results Boosting of the saccharification of p

Externí odkaz: https://doaj.org/article/6b05e199c5fb4002b7cd1b7d1d477a81

GO-PROMTO illuminates protein membrane topologies of glycan biosynthetic enzymes in the Golgi apparatus of living tissues.

Autor: Casper Søgaard, Anne Stenbæk, Sophie Bernard, Masood Hadi, Azeddine Driouich, Henrik Vibe Scheller, Yumiko Sakuragi

Publikováno v: PLoS ONE, Vol 7, Iss 2, p e31324 (2012)

The Golgi apparatus is the main site of glycan biosynthesis in eukaryotes. Better understanding of the membrane topology of the proteins and enzymes involved can impart new mechanistic insights into these processes. Publically available bioinformatic

Externí odkaz: https://doaj.org/article/523de6dbcfa64864a025a10ec4b41ed7

A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus

Autor: Henrik Vibe Scheller, Anne Stenbaek, Christian Have Lund, Yumiko Sakuragi, Randi Engelberth Rasmussen, Jennifer R. Bromley

Publikováno v: Journal of experimental botany, vol 66, iss 1
Journal of Experimental Botany
Lund, C H, Bromley, J R, Stenbæk, A, Rasmussen, R E, Scheller, H V & Sakuragi, Y 2015, ' A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus ', Journal of Experimental Botany, vol. 66, no. 1, pp. 85-97 . https://doi.org/10.1093/jxb/eru401
Lund, CH; Bromley, JR; Stenbæk, A; Rasmussen, RE; Scheller, HV; & Sakuragi, Y. (2015). A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus. Journal of Experimental Botany, 66(1), 85-97. doi: 10.1093/jxb/eru401. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/0n90z5xt

Summary A reversible Renilla luciferase protein complementation assay for rapid identification of protein–protein interactions revealed the existence of an interaction network involved in xyloglucan biosynthesis in the Golgi apparatus in plants.