Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells.

Autor: Angela Schoolmeesters, Teresa Eklund, Devin Leake, Annaleen Vermeulen, Queta Smith, Shelley Force Aldred, Yuriy Fedorov

Publikováno v: PLoS ONE, Vol 4, Iss 5, p e5605 (2009)

BACKGROUND:Mesenchymal stem (MS) cells are excellent candidates for cell-based therapeutic strategies to regenerate injured tissue. Although human MS cells can be isolated from bone marrow and directed to differentiate by means of an osteogenic pathw

Externí odkaz: https://doaj.org/article/b9d31ec996a549c887f1acd3e264fa91

Functional Screening Identifies Human miRNAs that Modulate Adenovirus Propagation in Prostate Cancer Cells

Autor: Annaleen Vermeulen, Daoud Sie, Jasmina Hodzic, Victor W. van Beusechem

Publikováno v: Hodzic, J, Sie, D, Vermeulen, A & Van Beusechem, V W 2017, ' Functional Screening Identifies Human miRNAs that Modulate Adenovirus Propagation in Prostate Cancer Cells ', Human Gene Therapy, vol. 28, no. 9, pp. 766-780 . https://doi.org/10.1089/hum.2016.143
Human Gene Therapy, 28(9), 766-780. Mary Ann Liebert Inc.

Oncolytic adenoviruses represent a novel class of anticancer agents. Their efficacy in killing cancer cells is variable, suggesting that there is room for improvement. Host miRNAs have been shown to play important roles in susceptibility of cells to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1af590b6fd83558e3c49afd0613ee141
https://doi.org/10.1089/hum.2016.143

Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing

Autor: Anja van Brabant Smith, Žaklina Strezoska, Annaleen Vermeulen, Kaizhang He, Melissa L. Kelley

Publikováno v: Journal of Biotechnology. 233:74-83

The CRISPR-Cas9 system has become the most popular and efficient method for genome engineering in mammalian cells. The Streptococcus pyogenes Cas9 nuclease can function with two types of guide RNAs: the native dual crRNA and tracrRNA (crRNA:tracrRNA)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::346e700aeb9f5811106b594536e6f592
https://doi.org/10.1016/j.jbiotec.2016.06.011

The Power Decoder Simulator for the Evaluation of Pooled shRNA Screen Performance

Autor: Joshua Stahl, Michael Banos, Amanda Birmingham, Anja van Brabant Smith, Annaleen Vermeulen, Abel Licon, Žaklina Strezoska, Jesse Stombaugh, Sarah B. Anderson

Publikováno v: Journal of Biomolecular Screening

RNA interference screening using pooled, short hairpin RNA (shRNA) is a powerful, high-throughput tool for determining the biological relevance of genes for a phenotype. Assessing an shRNA pooled screen’s performance is difficult in practice; one c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::116179eb7d790e18f1aefadaa2a68b4e
https://doi.org/10.1177/1087057115576715

Abstract 2761: Development of synthetic guide RNA libraries for CRISPR-mediated transcriptional activation screening for gain-of-function studies

Autor: Maren M. Gross, Matthew R. Perkett, Anja van Brabant Smith, Zaklina Strezoska, Shawn McClelland, Sarah Michelle Dickerson, Annaleen Vermeulen, Elena Maksimova, Eldon T. Chou

Publikováno v: Cancer Research. 79:2761-2761

Functional gene analysis studies have been empowered by development of CRISPR-Cas9 gene knockout tools, however the CRISPR-Cas9 system has also been adapted for inhibition or activation of gene transcription. A nuclease-deactivated Cas9 (dCas9) can b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::51fc4dac25ac9064a4f8d7ebbb450d91
https://doi.org/10.1158/1538-7445.am2019-2761