Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Annabel K. Sangree"'
Autor:
Nazanin Esmaeili Anvar, Chenchu Lin, Xingdi Ma, Lori L. Wilson, Ryan Steger, Annabel K. Sangree, Medina Colic, Sidney H. Wang, John G. Doench, Traver Hart
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Genetic interactions mediate the emergence of phenotype from genotype, but technologies for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we identify background-independent paralog synthetic lethals fr
Externí odkaz:
https://doaj.org/article/c7711b8dde9c4e0a8d46f98bf7f711db
Autor:
Annabel K. Sangree, Audrey L. Griffith, Zsofia M. Szegletes, Priyanka Roy, Peter C. DeWeirdt, Mudra Hegde, Abby V. McGee, Ruth E. Hanna, John G. Doench
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here the authors make comparisons of numerous Cas9 variants, nominate options for base editing screens with denser c
Externí odkaz:
https://doaj.org/article/1a7462c71ec4497c818edebbbedb24ca
Autor:
W. Frank Lenoir, Micaela Morgado, Peter C. DeWeirdt, Megan McLaughlin, Audrey L. Griffith, Annabel K. Sangree, Marissa N. Feeley, Nazanin Esmaeili Anvar, Eiru Kim, Lori L. Bertolet, Medina Colic, Merve Dede, John G. Doench, Traver Hart
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
CRISPR-based knockout screens in cancer cells have suggested the existence of proliferation suppressor genes (PSG). Here, the authors develop an approach to systematically identify them, and reveal a PSG module involved in fatty acid synthesis and tu
Externí odkaz:
https://doaj.org/article/49f1eb14cd9f49cb88919c70c04a8f5c
Autor:
Peter C. DeWeirdt, Annabel K. Sangree, Ruth E. Hanna, Kendall R. Sanson, Mudra Hegde, Christine Strand, Nicole S. Persky, John G. Doench
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.
Externí odkaz:
https://doaj.org/article/892a16ea5f874784a2584fee9329b79a
Autor:
Nazanin Esmaeili Anvar, Chenchu Lin, Lori L. Wilson, Annabel K. Sangree, Xingdi Ma, Medina Colic, John G. Doench, Traver Hart
Genetic interactions mediate the emergence of phenotype from genotype, but initial technologies for multiplex genetic perturbation in mammalian cells suffer from inefficiency and are challenging to scale. Recent focus on paralog synthetic lethality i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::577f0648bbfefd6a04955707307165c9
https://doi.org/10.1101/2023.01.03.522655
https://doi.org/10.1101/2023.01.03.522655
Autor:
Megan McLaughlin, Medina Colic, Micaela Morgado, John G. Doench, Traver Hart, Peter C DeWeirdt, Eiru Kim, Audrey L Griffith, Nazanin Esmaeili Anvar, W. Frank Lenoir, Marissa N Feeley, Lori Bertolet, Merve Dede, Annabel K Sangree
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Nature Communications
Nature Communications
CRISPR knockout fitness screens in cancer cell lines reveal many genes whose loss of function causes cell death or loss of fitness or, more rarely, the opposite phenotype of faster proliferation. Here we demonstrate a systematic approach to identify
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::396f4e37d6ca5400494ad75fc4cf64f0
https://doaj.org/article/49f1eb14cd9f49cb88919c70c04a8f5c
https://doaj.org/article/49f1eb14cd9f49cb88919c70c04a8f5c
Autor:
J. Keith Joung, Peter C DeWeirdt, Marissa N Feeley, Kendall R Sanson, Teng Teng, Xuewen Pan, Audrey L Griffith, John G. Doench, Benjamin P. Kleinstiver, Mudra Hegde, Ruth E Hanna, Samantha M Borys, Alan Huang, Christine Strand, Annabel K Sangree
Publikováno v:
Nature biotechnology
Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations because they can process multiple guide RNAs expressed as a single transcript and subsequently cleave target DNA. However, their widespread adoption has lagged
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52a28c50d1dc51154682a775da11902b
https://doi.org/10.1038/s41587-020-0600-6
https://doi.org/10.1038/s41587-020-0600-6
Autor:
Z. M. Szegletesh, Mudra Hegde, Priyanka Roy, John G. Doench, Peter C DeWeirdt, Audrey L Griffith, Annabel K Sangree, Ruth E Hanna, A. V. McGee
Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here, we benchmark PAM preferences, on-target activity, and off-target susceptibility of 11 variants of SpCas9 in ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4363f134bb4b62693c72752e8b5c47ba
https://doi.org/10.1101/2021.08.18.456848
https://doi.org/10.1101/2021.08.18.456848
Autor:
Annabel K. Sangree, Audrey L. Griffith, Zsofia M. Szegletes, Priyanka Roy, Peter C. DeWeirdt, Mudra Hegde, Abby V. McGee, Ruth E. Hanna, John G. Doench
Publikováno v:
Nature communications. 13(1)
Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here, we assess the activity and specificity of WT-Cas9 and 10 SpCas9 variants by benchmarking their PAM preferences
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd0733bbda777c8d9b98a36b53ea92ac
https://pubmed.ncbi.nlm.nih.gov/35288574
https://pubmed.ncbi.nlm.nih.gov/35288574
Autor:
Eiru Kim, Merve Dede, Micaela Morgado, Nazanin Esmaeili Anvar, Peter C DeWeirdt, W. Frank Lenoir, Medina Colic, Audrey L Griffith, Annabel K Sangree, Traver Hart, Lori Bertolet, Marissa N Feeley, John G. Doench, Megan McLaughlin
CRISPR knockout screens in hundreds of cancer cell lines have revealed a substantial number of context-specific essential genes that, when associated with a biomarker such as lineage or oncogenic mutation, offer candidate tumor-specific vulnerabiliti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::08081b8d4e361490c876c6dddb044347
https://doi.org/10.1101/2020.10.08.332023
https://doi.org/10.1101/2020.10.08.332023