Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Annabel Guichard"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract CRISPR-interference (CRISPRi), a highly effective method for silencing genes in mammalian cells, employs an enzymatically dead form of Cas9 (dCas9) complexed with one or more guide RNAs (gRNAs) with 20 nucleotides (nt) of complementarity to
Externí odkaz:
https://doaj.org/article/913069c903b94379a10027ebc9b198a9
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-1 (2024)
Externí odkaz:
https://doaj.org/article/788bb4e8e1b645bc9ac6cdd6c8abe8fe
Autor:
Annabel Guichard, Shenzhao Lu, Oguz Kanca, Daniel Bressan, Yan Huang, Mengqi Ma, Sara Sanz Juste, Jonathan C. Andrews, Kristy L. Jay, Marketta Sneider, Ruth Schwartz, Mei-Chu Huang, Danqing Bei, Hongling Pan, Liwen Ma, Wen-Wen Lin, Ankush Auradkar, Pranjali Bhagwat, Soo Park, Kenneth H. Wan, Takashi Ohsako, Toshiyuki Takano-Shimizu, Susan E. Celniker, Michael F. Wangler, Shinya Yamamoto, Hugo J. Bellen, Ethan Bier
Publikováno v:
Cell Reports, Vol 42, Iss 8, Pp 112842- (2023)
Summary: Development of effective therapies against SARS-CoV-2 infections relies on mechanistic knowledge of virus-host interface. Abundant physical interactions between viral and host proteins have been identified, but few have been functionally cha
Externí odkaz:
https://doaj.org/article/984e325c04524d81953603c94fb0a65a
Autor:
Bhagyashree Kaduskar, Raja Babu Singh Kushwah, Ankush Auradkar, Annabel Guichard, Menglin Li, Jared B. Bennett, Alison Henrique Ferreira Julio, John M. Marshall, Craig Montell, Ethan Bier
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Insecticide resistance (IR) poses a major global health challenge. Here, the authors generate common IR mutations in laboratory Drosophila strains and use a CRISPR-based allelic-drive to replace an IR allele with a susceptible wild-type counterpart,
Externí odkaz:
https://doaj.org/article/f9c5e9f5132e4a7aa564c5c7a2289e58
Autor:
Annabel Guichard, Tisha Haque, Marketta Bobik, Xiang-Ru S. Xu, Carissa Klanseck, Raja Babu Singh Kushwah, Mateus Berni, Bhagyashree Kaduskar, Valentino M. Gantz, Ethan Bier
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
Gene-drives use CRISPR-Cas9 to be transmitted in a super-Mendelian fashion. Here the authors develop an allelic-drive for selective inheritance of a desired allele.
Externí odkaz:
https://doaj.org/article/d84a958ee4384c8680de46671dd3e198
Publikováno v:
iScience, Vol 23, Iss 2, Pp - (2020)
Summary: Clostridium difficile infections (CDIs) cause severe and occasionally life-threatening diarrhea. Hyper-virulent strains produce CDT, a toxin that ADP-ribosylates actin monomers and inhibits actin polymerization. We created transgenic Drosoph
Externí odkaz:
https://doaj.org/article/6e070d472ea2492aa8c0e6eae8f8f453
Autor:
Annabel Guichard, Prashant Jain, Mahtab Moayeri, Ruth Schwartz, Stephen Chin, Lin Zhu, Beatriz Cruz-Moreno, Janet Z Liu, Bernice Aguilar, Andrew Hollands, Stephen H Leppla, Victor Nizet, Ethan Bier
Publikováno v:
PLoS Pathogens, Vol 13, Iss 9, p e1006603 (2017)
Various bacterial toxins circumvent host defenses through overproduction of cAMP. In a previous study, we showed that edema factor (EF), an adenylate cyclase from Bacillus anthracis, disrupts endocytic recycling mediated by the small GTPase Rab11. As
Externí odkaz:
https://doaj.org/article/7da69dcd7e13456c9f96af4371105fec
Autor:
Margery G Smelkinson, Annabel Guichard, John R Teijaro, Meghana Malur, Maria Eugenia Loureiro, Prashant Jain, Sundar Ganesan, Elina I Zúñiga, Robert M Krug, Michael B Oldstone, Ethan Bier
Publikováno v:
PLoS Pathogens, Vol 13, Iss 8, p e1006588 (2017)
The multifunctional NS1 protein of influenza A viruses suppresses host cellular defense mechanisms and subverts other cellular functions. We report here on a new role for NS1 in modifying cell-cell signaling via the Hedgehog (Hh) pathway. Genetic epi
Externí odkaz:
https://doaj.org/article/8296206c2f914e29bb8799c937494a0b
Autor:
Ethan Bier, Annabel Guichard
Publikováno v:
Disease Models & Mechanisms, Vol 5, Iss 1, Pp 48-61 (2012)
Many of the cellular mechanisms underlying host responses to pathogens have been well conserved during evolution. As a result, Drosophila can be used to deconstruct many of the key events in host-pathogen interactions by using a wealth of well-develo
Externí odkaz:
https://doaj.org/article/9b6fe82e127c49ddbee608437274a494
Autor:
Sitara Roy, Sara Sanz Juste, Marketta Sneider, Ankush Auradkar, Carissa Klanseck, Zhiqian Li, Alison Henrique Ferreira Julio, Victor Lopez del Amo, Ethan Bier, Annabel Guichard
Publikováno v:
Science Advances. 8
Repair of double-strand breaks (DSBs) in somatic cells is primarily accomplished by error-prone nonhomologous end joining and less frequently by precise homology-directed repair preferentially using the sister chromatid as a template. Here, a Drosoph