Biosynthesis of Antifungal Solanimycin May Involve an Iterative Nonribosomal Peptide Synthetase Module

Autor: Annabel C. Murphy, Matthew Corney, Rita E. Monson, Miguel A. Matilla, George P. C. Salmond, Finian J. Leeper

Publikováno v: ACS Chemical Biology. 18:1148-1157

Dickeya solani, a plant-pathogenic bacterium, produces solanimycin, a potent hybrid polyketide/nonribosomal peptide (PKS/NRPS) anti-fungal compound. The biosynthetic gene cluster responsible for synthesis of this compound has been identified. Because

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80fd766d2968a5be47ff426d36d8fdae
https://doi.org/10.1021/acschembio.2c00947

A vitamin K-dependent carboxylase orthologue is involved in antibiotic biosynthesis

Autor: Annabel C. Murphy, Peter F. Leadlay, Markiyan Samborskyy, Ying Zhuo, Brian J. C. Law, Daniel Francis, Yingxin Zhang, Michael Winn, Jason Micklefield, Lujing Ren

Publikováno v: Law, B, Zhuo, Y, Winn, M, Francis, D, Zhang, Y, Samborskyy, M, Murphy, A, Ren, L, Leadlay, P F & Micklefield, J 2018, ' A vitamin K-dependent carboxylase orthologue is involved in antibiotic biosynthesis ', Nature Catalysis, vol. 1, pp. 977-984 . https://doi.org/10.1038/s41929-018-0178-2

Vitamin K-dependent carboxylase (VKDC) enzymes modify glutamate residues in mammalian vitamin K-dependent proteins, generating γ-carboxyglutamic acids with malonate moieties that mediate important physiological responses such as blood coagulation. P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb90ea95c5b43088e7553ecc5aab5c5f
https://pure.manchester.ac.uk/ws/files/81218400/41929_2018_178_Author.pdf

Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation

Autor: Annabel C, Murphy, Hui, Hong, Steve, Vance, R William, Broadhurst, Peter F, Leadlay

Publikováno v: Chemical communications (Cambridge, England). 52(54)

An in vitro model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe the apparent substrate tolerance of ketosynthase domains of the mycolactone polyketide synthase. A specific residue change was identifie

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https://pubmed.ncbi.nlm.nih.gov/27307197

Evolving Trends in the Dereplication of Natural Product Extracts. 2. The Isolation of Chrysaibol, an Antibiotic Peptaibol from a New Zealand Sample of the Mycoparasitic Fungus Sepedonium chrysospermum

Autor: Maya I. Mitova, Anthony L. J. Cole, John W. Blunt, Gerhard Lang, Annabel C. Murphy, Murray H. G. Munro, Gill Ellis

Publikováno v: Journal of Natural Products. 71:1600-1603

By the application of an HPLC bioactivity profiling/microtiter technique in conjunction with capillary NMR instrumentation and access to the AntiMarin database the conventional evaluation/isolation dereplication/characterization procedures can be dra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::045e0b5a06a9e39d2a979726d792c680
https://doi.org/10.1021/np800221b

Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp SANK 73390

Autor: Annabel C. Murphy, Christine L. Willis, Russell J. Cox, Shu-Shan Gao, Zhongshu Song, Daisuke Fukuda, Christopher M. Thomas, John Crosby, Simon Carobene, Thomas J. Simpson, Matthew P. Crump, Li-Chen Han, Joanne Hothersall

Publikováno v: Murphy, A C, Gao, S-S, Han, L-C, Carobene, S, Fukuda, D, Song, Z, Hothersall, J, Cox, R J, Crosby, J, Crump, M P, Thomas, C M, Willis, C L & Simpson, T J 2014, ' Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp SANK 73390 ', Chemical Science, vol. 5, no. 1, pp. 397-402 . https://doi.org/10.1039/c3sc52281d

The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09306524968c8c1df3d5ba2e55198281
https://research-information.bris.ac.uk/ws/files/88017654/c3sc52281d.pdf