Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins

Autor: M. Moradi Barzadd, Anna-Luisa Volk, Diane Hatton, Raymond Field, Aman Mebrahtu, Paul Varley, R. G. Roth, Veronique Chotteau, N. Wistabacka, Fredrik Edfors, Chih-Chung Kuo, Magdalena Malm, Nathan E. Lewis, Ronia Razavi, T. Graslund, Johan Rockberg, David Kotol, M. Lunqvist

Publikováno v: Metabolic Engineering. 72:171-187

Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify metabolic engineering targets limiting expression of recombinant human proteins through a sys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc0046abd38c8f4d0e6970b315d289d7
https://doi.org/10.1016/j.ymben.2022.03.009

High throughput generation of a resource of the human secretome in mammalian cells

Autor: Mathias Uhlén, Malin Westin, Andreas Hober, LanLan Xu, Björn Forsström, Bjørn G. Voldborg, Faranak Bidad, Siri Ekblad, Delaram Afshari, Lovisa Holmberg Schiavone, Hanna Tegel, Kalle von Feilitzen, Johan Rockberg, Jenny Ottosson Takanen, Fredrik Edfors, Sophia Hober, Anne-Sophie Svensson, Melanie Dannemeyer, Henric Enstedt, Magnus Lundqvist, Sinead Knight, Sven Göpel, Martin Zwahlen, Mona Moradi, Anna Berling, Rick Davies, Anna-Luisa Volk, Åsa Sivertsson, Lorenz M. Mayr, Sara Kanje

Publikováno v: Tegel, H, Dannemeyer, M, Kanje, S, Sivertsson, Å, Berling, A, Svensson, A S, Hober, A, Enstedt, H, Volk, A L, Lundqvist, M, Moradi, M, Afshari, D, Ekblad, S, Xu, L L, Westin, M, Bidad, F, Schiavone, L H, Davies, R, Mayr, L M, Knight, S, Göpel, S O, Voldborg, B G, Edfors, F, Forsström, B, von Feilitzen, K, Zwahlen, M, Rockberg, J, Takanen, J O, Uhlén, M & Hober, S 2020, ' High throughput generation of a resource of the human secretome in mammalian cells ', New Biotechnology, vol. 58, pp. 45-54 . https://doi.org/10.1016/j.nbt.2020.05.002

The proteins secreted by human tissues and blood cells, the secretome, are important both for the basic understanding of human biology and for identification of potential targets for future diagnosis and therapy. Here, a high-throughput mammalian cel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac3c343bc5f3f5e43bfd18155a712f5c
https://pubmed.ncbi.nlm.nih.gov/32502629

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

Autor: Mathias Uhlén, Niklas Thalén, Henning Gram Hansen, Per-Åke Nygren, Johan Rockberg, Anna-Luisa Volk, Eric von Otter, Magnus Lundqvist

Publikováno v: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
Lundqvist, M, Thalén, N, Volk, A L, Hansen, H G, von Otter, E, Nygren, P-Å, Uhlén, M & Rockberg, J 2019, ' Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion ', Scientific Reports, vol. 9, no. 1, 310 . https://doi.org/10.1038/s41598-018-36559-x

Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1–10 and GFP 11, has previously provided a convenient approach to measur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06b0b4e06c9a0fb55572a1d7639cd551
http://europepmc.org/articles/PMC6342966

Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains

Autor: Anna-Luisa, Volk, Johan, Rockberg

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1785

Knowledge of the exquisite-binding surface of an antibody on its target protein is of great value, in particular for therapeutic antibodies for understanding method of action and for stratification of patients carrying the necessary epitope for desir

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::704aada6e4cdec92e5579ac114e89443
https://pubmed.ncbi.nlm.nih.gov/29714018

Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries

Autor: Johan Rockberg, Anna-Luisa Volk, Francis Jingxin Hu

Publikováno v: Epitope Mapping Protocols ISBN: 9781493978397

The unique property of specific high affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. Hence, knowledge of the precise binding site (epitope) of antibodies on the target protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d0147a4641399a8694df86fcd09efdae
https://doi.org/10.1007/978-1-4939-7841-0_10