Mechanical loading of desmosomes depends on the magnitude and orientation of external stress

Autor: Andrew J. Price, Anna-Lena Cost, Hanna Ungewiß, Jens Waschke, Alexander R. Dunn, Carsten Grashoff

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)

Desmosomes are intercellular adhesion complexes that connect the intermediate filament cytoskeletons of neighboring cells but direct evidence for their load-bearing nature is lacking. Here the authors develop FRET-based tension sensors to measure the

Externí odkaz: https://doaj.org/article/2215fbe56cc84a20a528fc6fe6ae67ab

Mechanical loading of desmosomes depends on the magnitude and orientation of external stress

Autor: Alexander R. Dunn, Jens Waschke, Hanna Ungewiß, Andrew Price, Anna-Lena Cost, Carsten Grashoff

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
Nature Communications

Desmosomes are intercellular adhesion complexes that connect the intermediate filament cytoskeletons of neighboring cells, and are essential for the mechanical integrity of mammalian tissues. Mutations in desmosomal proteins cause severe human pathol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a5eb23d3db79fe93989da703382ef85
http://link.springer.com/article/10.1038/s41467-018-07523-0

A small proportion of Talin molecules transmit forces at developing muscle attachments in vivo

Autor: Frank Schnorrer, Anna-Lena Cost, Carsten Grashoff, Thomas Weidemann, Sandra B. Lemke

Publikováno v: PLoS Biology
PLoS Biology, Public Library of Science, 2019, 17 (3), pp.e3000057. ⟨10.1371/journal.pbio.3000057⟩
PLoS Biology, 2019, 17 (3), pp.e3000057. ⟨10.1371/journal.pbio.3000057⟩
PLoS Biology, Vol 17, Iss 3, p e3000057 (2019)

Cells in developing organisms are subjected to particular mechanical forces that shape tissues and instruct cell fate decisions. How these forces are sensed and transmitted at the molecular level is therefore an important question, one that has mainl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfa277b5f234c61dbbbec7a864a65118
https://hal.archives-ouvertes.fr/hal-02368074/document

The Piconewton Force Awakens: Quantifying Mechanics in Cells

Autor: Carsten Grashoff, Anna-Lena Cost, Andrea Freikamp

Publikováno v: Trends in Cell Biology

The development of calibrated Forster resonance energy transfer (FRET)-based tension sensors has allowed the first analyses of mechanical processes with piconewton (pN) sensitivity in cells. Here, we introduce the working principle of this emerging m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b510aa3a9901944f4d55fc39249fbc19
https://pubmed.ncbi.nlm.nih.gov/27544876

How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors

Autor: Anna Chrostek-Grashoff, Carsten Grashoff, Anna-Lena Cost, Pia Ringer

Publikováno v: Cellular and Molecular Bioengineering

The ability of cells to sense and respond to mechanical forces is central to a wide range of biological processes and plays an important role in numerous pathol- ogies. The molecular mechanisms underlying cellular mech- anotransduction, however, have

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67fef57d5376c24b3824d2662efcaed4
https://hdl.handle.net/11858/00-001M-0000-0025-A9A7-111858/00-001M-0000-0025-A9A5-5