Emerging approaches for detection of methylation sites in RNA

Autor: Anna Ovcharenko, Andrea Rentmeister

Publikováno v: Open Biology, Vol 8, Iss 9 (2018)

RNA methylations play a significant regulatory role in diverse biological processes. Although the transcriptome-wide discovery of unknown RNA methylation sites is essential to elucidate their function, the development of a bigger variety of detection

Externí odkaz: https://doaj.org/article/10516a901bf14e809d346d60ebc2ef33

MePMe-seq: Antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing

Autor: Katja Hartstock, Anna Ovcharenko, Nadine A. Kueck, Petr Spacek, Nicolas V. Cornelissen, Sabine Hüwel, Christoph Dieterich, Andrea Rentmeister

Internal modifications of mRNA have emerged as widespread and versatile regulatory mechanism to control gene expression at the post-transcriptional level. Current insights rely on the ability to make a modified nucleoside amenable to sequencing. Most

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fd240fdc78cb727a5f41e55a036cccb6
https://doi.org/10.1101/2022.03.16.484494

Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions

Autor: Nicolas V. Cornelissen, Aileen Peters, Andrea Rentmeister, Freideriki Michailidou, Rohit Singh, Daniel Kümmel, Nils Klöcker, Anna Ovcharenko

Publikováno v: Angewandte Chemie (International Ed. in English)
Angewandte Chemie. International Edition, 60 (1)
Angewandte Chemie / International edition 60(1), 480-485 (2020). doi:10.1002/anie.202012623
Angewandte Chemie International Edition

Methylation and demethylation of DNA, RNA and proteins has emerged as a major regulatory mechanism. Studying the function of these modifications would benefit from tools for their site‐specific inhibition and timed removal. S‐Adenosyl‐L‐methi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::643f207a0e82f52ff52e3124f60b48b2
http://europepmc.org/articles/PMC7839696

Chemoenzymatic labeling of RNA to enrich, detect and identify methyltransferase-target sites

Autor: Nadine A, Kueck, Anna, Ovcharenko, Katja, Hartstock, Nicolas V, Cornelissen, Andrea, Rentmeister

Publikováno v: Methods in enzymology. 658

The RNA methyltransferase (MTase) complex METTL3-METTL14 transfers methyl groups from S-adenosyl-l-methionine (AdoMet) to the N

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::53c515cacc132d8f6323dce528f0aa79
https://pubmed.ncbi.nlm.nih.gov/34517946

Chemoenzymatic labeling of RNA to enrich, detect and identify methyltransferase-target sites

Autor: Nicolas V. Cornelissen, Nadine A. Kueck, Andrea Rentmeister, Anna Ovcharenko, Katja Hartstock

Publikováno v: Methods in Enzymology ISBN: 9780128235850

The RNA methyltransferase (MTase) complex METTL3-METTL14 transfers methyl groups from S-adenosyl- l -methionine (AdoMet) to the N6-position of adenosines within its consensus sequence, the DR A CH motif (D = A, G, U; R = A, G; H = A, C, U). Interesti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f447b8c6649c889f9a0ace94e4d5552d
https://doi.org/10.1016/bs.mie.2021.06.006