Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Anna Noble"'
Autor:
James N. Cobley, Anna Noble, Eduardo Jimenez-Fernandez, Manuel-Thomas Valdivia Moya, Matthew Guille, Holger Husi
Publikováno v:
Redox Biology, Vol 26, Iss , Pp - (2019)
Using non-reducing Western blotting to assess protein thiol redox state is challenging because most reduced and oxidised forms migrate at the same molecular weight and are, therefore, indistinguishable. While copper catalysed Click chemistry can be u
Externí odkaz:
https://doaj.org/article/d18f44a516b8445893e1d7c561771be6
Autor:
Marko Horb, Marcin Wlizla, Anita Abu-Daya, Sean McNamara, Dominika Gajdasik, Takeshi Igawa, Atsushi Suzuki, Hajime Ogino, Anna Noble, Centre de Ressource Biologique Xenope team in France, Jacques Robert, Christina James-Zorn, Matthew Guille, Morgane Nicolas, Thomas Lafond, Daniel Boujard, Yann Audic, Brigitte Guillet
Publikováno v:
Frontiers in Physiology, Vol 10 (2019)
Two species of the clawed frog family, Xenopus laevis and X. tropicalis, are widely used as tools to investigate both normal and disease-state biochemistry, genetics, cell biology, and developmental biology. To support both frog specialist and non-sp
Externí odkaz:
https://doaj.org/article/517a3ba5a3ee446b88be700ee1c23185
Publikováno v:
Antioxidants, Vol 9, Iss 3, p 215 (2020)
Oocytes are postulated to repress the proton pumps (e.g., complex IV) and ATP synthase to safeguard mitochondrial DNA homoplasmy by curtailing superoxide production. Whether the ATP synthase is inhibited is, however, unknown. Here we show that: oligo
Externí odkaz:
https://doaj.org/article/68dddcf566334cab8f67a9824df33afb
Autor:
Takeshi Igawa, Ai Watanabe, Atsushi Suzuki, Akihiko Kashiwagi, Keiko Kashiwagi, Anna Noble, Matt Guille, David E Simpson, Marko E Horb, Tamotsu Fujii, Masayuki Sumida
Publikováno v:
PLoS ONE, Vol 10, Iss 7, p e0133963 (2015)
The Western clawed frog, Xenopus tropicalis, is a highly promising model amphibian, especially in developmental and physiological research, and as a tool for understanding disease. It was originally found in the West African rainforest belt, and was
Externí odkaz:
https://doaj.org/article/4b0ce78a35d7463cab875aab594fc0ea
Publikováno v:
Free Radical Biology and Medicine. 181:118-129
Unravelling how reactive oxygen species regulate fundamental biological processes is hampered by the lack of an accessible microplate technique to quantify target-specific protein thiol redox state in percentages and moles. To meet this unmet need, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::062ef63e8694c43071f7e5831988dd6e
https://doi.org/10.1016/j.freeradbiomed.2022.01.023
https://doi.org/10.1016/j.freeradbiomed.2022.01.023
Publikováno v:
Free Radical Biology and Medicine. 174:272-280
Measuring protein thiol redox state is central to understanding redox signalling in health and disease. The lack of a microplate assay to measure target specific protein thiol redox state rate-limits progress on accessibility grounds: redox proteomic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ad885b08aa8dfd6e7831d4812843796
https://doi.org/10.1016/j.freeradbiomed.2021.08.018
https://doi.org/10.1016/j.freeradbiomed.2021.08.018
Publikováno v:
Cold Spring Harbor protocols. 2022(6)
Transgenic frogs can be very efficiently generated using I-SceI meganuclease, a nuclease with an 18-bp recognition site. The desired transgene must be flanked by I-SceI sites, in either a plasmid or a polymerase chain reaction (PCR) product. After a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c8d1cd2f29fbf2f15755843ec6ece064
https://pubmed.ncbi.nlm.nih.gov/35135888
https://pubmed.ncbi.nlm.nih.gov/35135888
Publikováno v:
Cold Spring Harbor protocols. 2022(2)
The cryopreservation of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::31e17631e25a0f88d9648748c6e748a3
https://pubmed.ncbi.nlm.nih.gov/34531328
https://pubmed.ncbi.nlm.nih.gov/34531328
An accessible, time- and cost-efficient microplate assay to quantify protein thiol redox state in percentages and moles relative to the thiol proteome (i.e., context) and other targets (i.e., array mode) would be invaluable for understanding how prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2bb142029acc5e306fc6b971221a2953
https://doi.org/10.21203/rs.3.rs-847650/v1
https://doi.org/10.21203/rs.3.rs-847650/v1
Publikováno v:
Antioxidants
Cobley, J, Noble, A, Bessell, R, Guille, M & Husi, H 2020, ' Reversible thiol oxidation inhibits the mitochondrial ATP synthase in xenopus laevis oocytes ', Antioxidants, vol. 9, no. 3, 215 . https://doi.org/10.3390/antiox9030215
Volume 9
Issue 3
Antioxidants, Vol 9, Iss 3, p 215 (2020)
Cobley, J, Noble, A, Bessell, R, Guille, M & Husi, H 2020, ' Reversible thiol oxidation inhibits the mitochondrial ATP synthase in xenopus laevis oocytes ', Antioxidants, vol. 9, no. 3, 215 . https://doi.org/10.3390/antiox9030215
Volume 9
Issue 3
Antioxidants, Vol 9, Iss 3, p 215 (2020)
Oocytes are postulated to repress the proton pumps (e.g., complex IV) and ATP synthase to safeguard mitochondrial DNA homoplasmy by curtailing superoxide production. Whether the ATP synthase is inhibited is, however, unknown. Here we show that: oligo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::132a0a9ec0540d7a0bc18b44c5e7479e
http://europepmc.org/articles/PMC7139892
http://europepmc.org/articles/PMC7139892