Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification

Autor: Anna Maekiniemi, Robert H. Singer, Evelina Tutucci

Publikováno v: Data in Brief, Vol 30, Iss , Pp 105511- (2020)

Single-molecule fluorescent in situ hybridization (smFISH) has emerged as a powerful technique that allows one to localize and quantify the absolute number of mRNAs in single cells. In combination with immunofluorescence (IF), smFISH can be used to c

Externí odkaz: https://doaj.org/article/74dbe418aeb940bb8d3e79baf752918f

Cyclin CLB2 mRNA localization determines efficient protein synthesis to orchestrate bud growth and cell cycle progression

Autor: Evelina Tutucci, Anna Maekiniemi, Jacky L. Snoep, Markus Seiler, Kelly van Rossum, David D. van Niekerk, Philipp Savakis, Kathi Zarnack, Robert H. Singer

mRNA localization to subcellular compartments has been reported across all kingdoms of life and it is generally believed to promote asymmetric protein synthesis and localization. In striking contrast to previous observations, we show that in S. cerev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::900f8a57efe136beedb635c5a5002b88
https://doi.org/10.1101/2022.03.01.481833

Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification

Autor: Evelina Tutucci, Robert H. Singer, Anna Maekiniemi

Publikováno v: Data in brief, 30:105511, 1-11. Elsevier BV
Data in Brief, Vol 30, Iss, Pp 105511-(2020)
Maekiniemi, A, Singer, R H & Tutucci, E 2020, ' Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae : Dataset and quantification ', Data in brief, vol. 30, 105511, pp. 1-11 . https://doi.org/10.1016/j.dib.2020.105511

Single-molecule fluorescent in situ hybridization (smFISH) has emerged as a powerful technique that allows one to localize and quantify the absolute number of mRNAs in single cells. In combination with immunofluorescence (IF), smFISH can be used to c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1762f10a560149c0bd5cbab3449b7d1b
https://research.vu.nl/en/publications/e4874692-9993-4cb5-a8e7-1ff3eece670f