Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Anna Hemming"'
Publikováno v:
Journal of Virological Methods. 76:87-100
HIV-1 gp120 is heavily glycosylated containing 24 N-glycosylation sites, and this makes elucidation of the significance of glycans at individual glycosylation sites a difficult task. A procedure is described where a complex mixture of biologically ra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc3d27514f9f57894d8b1fa354e60d9a
https://doi.org/10.1016/s0166-0934(98)00127-x
https://doi.org/10.1016/s0166-0934(98)00127-x
Autor:
Sigvard Olofsson, Anders Bolmstedt, John-Erik Stig Hansen, Anna Hemming, Gregers J. Gram, A. Ricksten, B. Losman
Publikováno v:
Archives of Virology. 141:2139-2151
We have constructed a mutated infectious HIV variant lacking the signals for addition of three N-linked glycans situated in the V4, C4 and V5 regions of HIV gp120. When comparing mutated virus with wildtype virus we found essentially no differences i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::410b01c43169ca2e25a451049dfb2d31
https://doi.org/10.1007/bf01718221
https://doi.org/10.1007/bf01718221
Autor:
S. L. Hu, Anna Hemming, B. Travis, Anders Bolmstedt, Sigvard Olofsson, Britt Jansson, John-Erik Stig Hansen
Publikováno v:
Archives of Virology. 134:335-344
Site-directed mutagenesis was used to study the biological significance of three N-linked glycans (linked to Asn406, Asn448, and Asn463), situated in the CD4-binding region of gp120. Mutagenesis was carried out in a phage M13 system, and the mutated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::372eafd12ab73f562d3396f1b294305d
https://doi.org/10.1007/bf01310571
https://doi.org/10.1007/bf01310571
Autor:
John-Erik Stig Hansen, Britt Jansson, Anna Hemming, Lennart Åkerblom, Gregers J. Gram, S. Olofsson, Anders Bolmstedt, J. O. Nielsen
Publikováno v:
Archives of Virology. 139:253-261
Glycosylation is necessary for HIV-1 gp120 to attain a functional conformation, and individual N-linked glycans of gp120 are important, but not essential, for replication of HIV-1 in cell culture. We have constructed a mutant HIV-1 infectious clone l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c23920f7fa0b5fc5691f74044c954c92
https://doi.org/10.1007/bf01310789
https://doi.org/10.1007/bf01310789
Publikováno v:
FEBS Letters. 286:13-17
Production of glycerol and a key enzyme in glycerol production, glycerol 3-phosphate dehydrogenase (NAD+) (GPD), was studied in Saccharomyces cerevisiae cultured in basal media or media of high salinity, with glucose, raffinose or ethanol as the sole
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc1bdd9d41d2281c1cd355748cd7169f
https://doi.org/10.1016/0014-5793(91)80930-2
https://doi.org/10.1016/0014-5793(91)80930-2
Autor:
Shiu Lok Hu, Bror Morein, Sigrid Sjölander, Sigvard Olofsson, Anders Bolmstedt, Anna Hemming, Lennart Åkerblom, John-Erik Stig Hansen
Publikováno v:
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association. 12(3)
One of the functions of N-linked glycans of viral glycoproteins is protecting otherwise accessible neutralization epitopes of the viral envelope from neutralizing antibodies. The aim of the present study was to explore the possibility to obtain a mor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a2dcb33f9d7c00fef52df191a67798f
https://pubmed.ncbi.nlm.nih.gov/8673525
https://pubmed.ncbi.nlm.nih.gov/8673525
Autor:
Pia Berntsson, Sigvard Olofsson, Hans Wigzell, Jeffrey P. C. Lin, Anders Bolmstedt, Anna Hemming, Per Flodby, Shiu-Lok Hu, Theta Tsu, Bruce Travis, Jeffrey Ledbetter
Publikováno v:
The Journal of general virology. 72
Site-directed mutagenesis was used to study the biological significance of a disulphide bridge and two N-linked oligosaccharides in the CD4-binding region of the envelope glycoproteins of human immunodeficiency virus type 1. Mutagenesis was performed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b34962e65fffe29c350020ae4239ed2a
https://pubmed.ncbi.nlm.nih.gov/2045792
https://pubmed.ncbi.nlm.nih.gov/2045792
Publikováno v:
Archives of Virology. 107:301-305
By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M 13 for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::408532e317446b69103f73e99e40d11c
https://doi.org/10.1007/bf01317925
https://doi.org/10.1007/bf01317925
Autor:
L. Lundberg, M. Gidlund, Sigvard Olofsson, P. Flodby, Anna Hemming, H. Wigzell, Anders Bolmstedt
Publikováno v:
Archives of virology. 109(3-4)
A DNA fragment encoding the CD4-binding region of human immunodeficiency virus type 1 (HIV) gp 120 was excised from an SV40-based expression vector containing gp 160, and subcloned into phage M13 for site-directed mutagenesis. Mutant vectors were con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fb5d6b51451259caf939b637c8bbab2
https://pubmed.ncbi.nlm.nih.gov/2558637
https://pubmed.ncbi.nlm.nih.gov/2558637