Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Anna, Moosmann"'
Publikováno v:
Journal of Chromatography A. 1236:90-96
In this manuscript we describe the two-step purification of a mono-PEGylated anti-epidermal growth factor receptor (EGFR) single-chain Fv. A weak cation exchanger was used for capture. Elution using arginine suppressed protein aggregation and allowed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4514ebe7d8b4bad97ac811cd75b7175
https://doi.org/10.1016/j.chroma.2012.03.004
https://doi.org/10.1016/j.chroma.2012.03.004
Publikováno v:
Bioconjugate Chemistry. 22:1545-1558
The mPEG-aldehyde PEGylation with two different PEG sizes and two proteins was experimentally determined with respect to yield, conversion, and selectivity. The kinetic behavior of these PEGylation reactions was simulated using a numerically solved s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46460d17fb1beb31116a27dbbd674b00
https://doi.org/10.1021/bc200090x
https://doi.org/10.1021/bc200090x
Publikováno v:
Journal of Chromatography A. 1217:209-215
The effect of PEGylation on cation exchange chromatography was studied with poly(ethylene glycol) of different chain lengths (5kDa, 10kDa and 30kDa) using lysozyme as a model system. A stable binding via reduction of a Schiff base was formed during r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d57b936f6242545780301e867a5707d
https://doi.org/10.1016/j.chroma.2009.11.031
https://doi.org/10.1016/j.chroma.2009.11.031
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1129
PEGylation is a common and highly accepted possibility for half-life prolongation of proteins by increasing the hydrodynamic size. The chromatographic purification of PEGylated protein, using PEG (poly-ethylene glycol) of different PEG chain lengths,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a01f0a4116b83feda7f4e05e2c2ae609
https://pubmed.ncbi.nlm.nih.gov/24648098
https://pubmed.ncbi.nlm.nih.gov/24648098
Publikováno v:
Methods in Molecular Biology ISBN: 9781627039765
PEGylation is a common and highly accepted possibility for half-life prolongation of proteins by increasing the hydrodynamic size. The chromatographic purification of PEGylated protein, using PEG (poly-ethylene glycol) of different PEG chain lengths,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4cd2a6826bbf5db0272c57a79efd6a3d
https://doi.org/10.1007/978-1-62703-977-2_37
https://doi.org/10.1007/978-1-62703-977-2_37
Publikováno v:
Journal of chromatography. A. 1217(28)
Dynamic binding capacities and resolution of PEGylated lysozyme derivatives with varying molecular weights of poly (ethylene) glycol (PEG) with 5 kDa, 10 kDa and 30 kDa for HIC resins and columns are presented. To find the optimal range for the opera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1f931659025a5cd5f1f72deb055c86c
https://pubmed.ncbi.nlm.nih.gov/20570270
https://pubmed.ncbi.nlm.nih.gov/20570270
Publikováno v:
Bioconjugate Chemistry; Aug2011, Vol. 22 Issue 8, p1545-1558, 14p