Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Ankush Auradkar"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract CRISPR-interference (CRISPRi), a highly effective method for silencing genes in mammalian cells, employs an enzymatically dead form of Cas9 (dCas9) complexed with one or more guide RNAs (gRNAs) with 20 nucleotides (nt) of complementarity to
Externí odkaz:
https://doaj.org/article/913069c903b94379a10027ebc9b198a9
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-1 (2024)
Externí odkaz:
https://doaj.org/article/788bb4e8e1b645bc9ac6cdd6c8abe8fe
Autor:
Annabel Guichard, Shenzhao Lu, Oguz Kanca, Daniel Bressan, Yan Huang, Mengqi Ma, Sara Sanz Juste, Jonathan C. Andrews, Kristy L. Jay, Marketta Sneider, Ruth Schwartz, Mei-Chu Huang, Danqing Bei, Hongling Pan, Liwen Ma, Wen-Wen Lin, Ankush Auradkar, Pranjali Bhagwat, Soo Park, Kenneth H. Wan, Takashi Ohsako, Toshiyuki Takano-Shimizu, Susan E. Celniker, Michael F. Wangler, Shinya Yamamoto, Hugo J. Bellen, Ethan Bier
Publikováno v:
Cell Reports, Vol 42, Iss 8, Pp 112842- (2023)
Summary: Development of effective therapies against SARS-CoV-2 infections relies on mechanistic knowledge of virus-host interface. Abundant physical interactions between viral and host proteins have been identified, but few have been functionally cha
Externí odkaz:
https://doaj.org/article/984e325c04524d81953603c94fb0a65a
Autor:
Bhagyashree Kaduskar, Raja Babu Singh Kushwah, Ankush Auradkar, Annabel Guichard, Menglin Li, Jared B. Bennett, Alison Henrique Ferreira Julio, John M. Marshall, Craig Montell, Ethan Bier
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Insecticide resistance (IR) poses a major global health challenge. Here, the authors generate common IR mutations in laboratory Drosophila strains and use a CRISPR-based allelic-drive to replace an IR allele with a susceptible wild-type counterpart,
Externí odkaz:
https://doaj.org/article/f9c5e9f5132e4a7aa564c5c7a2289e58
Autor:
Zhiqian Li, Nimi Marcel, Sushil Devkota, Ankush Auradkar, Stephen M. Hedrick, Valentino M. Gantz, Ethan Bier
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
CRISPR-based genetic elements can copy themselves across host genomes. Here the authors introduce CopyCatcher, a gene-drive related system for detecting and quantifying somatic gene conversion events.
Externí odkaz:
https://doaj.org/article/b94ecd06f7bb42bcb6d8bcbcc6d0a9f1
Autor:
Sitara Roy, Sara Sanz Juste, Marketta Sneider, Ankush Auradkar, Carissa Klanseck, Zhiqian Li, Alison Henrique Ferreira Julio, Victor Lopez del Amo, Ethan Bier, Annabel Guichard
Publikováno v:
Science Advances. 8
Repair of double-strand breaks (DSBs) in somatic cells is primarily accomplished by error-prone nonhomologous end joining and less frequently by precise homology-directed repair preferentially using the sister chromatid as a template. Here, a Drosoph
Publikováno v:
Science Advances
Description
Full replacement of D. melanogaster pb Hox locus with D. mimica pb locus produces mouthparts with similarities to D. mimica.
Hox genes determine positional codes along the head-to-tail axis. Here, we replaced the entire Drosophi
Full replacement of D. melanogaster pb Hox locus with D. mimica pb locus produces mouthparts with similarities to D. mimica.
Hox genes determine positional codes along the head-to-tail axis. Here, we replaced the entire Drosophi
Autor:
Nimi Marcel, Stephen M. Hedrick, Ethan Bier, Valentino M. Gantz, Sushil Devkota, Zhiqian Li, Ankush Auradkar
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature communications, vol 12, iss 1
Nature communications, vol 12, iss 1
CRISPR-based active genetic elements, or gene-drives, copied via homology-directed repair (HDR) in the germline, are transmitted to progeny at super-Mendelian frequencies. Active genetic elements also can generate widespread somatic mutations, but th
Autor:
Carissa Klanseck, Sara Sanz Juste, Stephanie R. Heimler, Valentino M. Gantz, Ankush Auradkar, John M. Marshall, Emily A. Bulger, Anna Buchman, Ethan Bier, Lauren Ashley Miller, Omar S. Akbari, Sarah Leahy, Jared B. Bennett, Xiang-Ru S. Xu
Publikováno v:
Molecular cell. 80(2)
CRISPR-Cas9-based gene drive systems possess the inherent capacity to spread progressively throughout target populations. Here we describe two self-copying (or active) guide RNA-only genetic elements, called e-CHACRs and ERACRs. These elements use Ca
Autor:
Raviranjan Kumar, Kalyani Palasamudrum, Ankush Auradkar, Sruthakeerthi Vuppala, Rohit Joshi, Maheshvari Chotaliya
Publikováno v:
Mechanisms of Development
The expression and regulation of Hox genes in developing central nervous system (CNS) lack important details like specific cell types where Hox genes are expressed and the transcriptional regulatory players involved in these cells. In this study we h