Activation mechanism and activity of globupain, a thermostable C11 protease from the Arctic Mid-Ocean Ridge hydrothermal system

Autor: Victoria Røyseth, Brianna M. Hurysz, Anna-Karina Kaczorowska, Sebastian Dorawa, Anita-Elin Fedøy, Hasan Arsın, Mateus Sá M. Serafim, Samuel A. Myers, Olesia Werbowy, Tadeusz Kaczorowski, Runar Stokke, Anthony J. O’Donoghue, Ida Helene Steen

Publikováno v: Frontiers in Microbiology, Vol 14 (2023)

Deep-sea hydrothermal vents offer unique habitats for heat tolerant enzymes with potential new enzymatic properties. Here, we present the novel C11 protease globupain, which was prospected from a metagenome-assembled genome of uncultivated Archaeoglo

Externí odkaz: https://doaj.org/article/66f3ac660b114410afbff83d2346c762

Going to extremes – a metagenomic journey into the dark matter of life

Autor: Alexandra Helleux, Håkon Dahle, Alexander Sczyrba, Karolina Kwiatkowska-Semrau, Anders Svensson, Ruoshi Zhang, Paul Terzian, Olesia Werbowy, Edda Olgudóttir, Gudmundur O. Hreggvidsson, Jonathan Vincent, Andrius Jasilionis, David Prangishvili, Thibaud Mas, Ehmke Pohl, Javier A. Linares-Pastén, Tobias Lutterman, Olafur H. Fridjonsson, Agata Jurczak-Kurek, Martin Steinegger, Arnthor Aevarsson, Justine Vanhalst, Mart Krupovic, Elisabet E. Gudmundsdottir, Sigurlaug Skirnisdottir, Milot Mirdita, Tadeusz Kaczorowski, Monika Szadkowska, Joanna Lange, Magdalena Plotka, Hildegard Watzlawick, François Enault, Magdalena Cichowicz-Cieślak, Sigmar K. Stefansson, Björn Walse, Jessica Louise Ray, Steffen A Lorentsen, Jörn Kalinowski, Slawomir Dabrowski, Josefin Ahlqvist, William Merre, Eric Olo Ndela, Lei Wang, Salam Al-Karadaghi, Sebastian Dorawa, Martin Welin, Francine Perler, Cathrine Pedersen, Tara Róbertsdóttir, Stefanie Freitag-Pohl, Anna-Karina Kaczorowska, Ruth-Anne Sandaa, Jérémy Courtin, Ilmur Jónsdóttir, Ewa Wons, Bjorn T. Adalsteinsson, Úlfur Áugúst Átlasson, Birkir Reynisson, Katrine Stange Overå, Christian Henke, Maria Håkansson, Julien Lebrat, Annika Jochheim, Mathilde Tourigny, Bas Vroling, Ying Liu, Eirin Glomsaker, Olav Lanes, Sigrídur Hjörleifdóttir, Sólveig K Pétursdóttir, Lukasz P. Kozlowski, Bernd Striberny, David Brandt, Agnieszka Morzywolek, Mickael Guérin, Julia Dusaucy, Ida Helene Steen, Clovis Galiez, Sigurd E Gundesø, J. Altenbuchner, Lilja Björk Jónsdóttir, Jørn Remi Henriksen, Katy A. S. Cornish, Tom van den Bergh, Eva Nordberg Karlsson, Johannes Söding, Hördur Gudmundsson, Hasan Arsin, Anita-Elin Fedøy, Emma J Tarrant, Samia Djeffane, Terese Solstad

Publikováno v: FEMS Microbiology Letters
FEMS Microbiology Letters, 2021, 368 (12), pp.fnab067. ⟨10.1093/femsle/fnab067⟩

The Virus-X-Viral Metagenomics for Innovation Value-project was a scientific expedition to explore and exploit uncharted territory of genetic diversity in extreme natural environments such as geothermal hot springs and deep-sea ocean ecosystems. Spec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5ace0dc1df1a5c289378af4e9b0cf30
https://hal-pasteur.archives-ouvertes.fr/pasteur-03698255

Structural and Functional Properties of Isocitrate Dehydrogenase from the Psychrophilic Bacterium Desulfotalea psychrophila Reveal a Cold-active Enzyme with an Unusual High Thermal Stability

Autor: Ida Helene Steen, Hanna-Kirsti S. Leiros, Nannan Yang, Anita-Elin Fedøy, Aurora Martinez

Publikováno v: Journal of Molecular Biology. 372:130-149

Isocitrate dehydrogenase (IDH) has been studied extensively due to its central role in the Krebs cycle, catalyzing the oxidative NAD(P)(+)-dependent decarboxylation of isocitrate to alpha-ketoglutarate and CO(2). Here, we present the first crystal st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1db4e481d169e0bb7414e0d7eae534b5
https://doi.org/10.1016/j.jmb.2007.06.040

Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD+-dependent homotetrameric enzyme

Autor: Runar Stokke, Ida Helene Steen, Solveig Karlsen, Anita-Elin Fedøy, Dominique Madern, Nils-Kåre Birkeland

Publikováno v: Archives of microbiology. 187(5)

The gene encoding isocitrate dehydrogenase (IDH) of Methylococcus capsulatus (McIDH) was cloned and overexpressed in Escherichia coli. The purified enzyme was NAD+-dependent with a thermal optimum for activity at 55-60 degrees C and an apparent midpo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a266b8cc4ae348395db6a8236b1a1c0
https://pubmed.ncbi.nlm.nih.gov/17160675