TOP1 CAD-seq: A protocol to map catalytically engaged topoisomerase 1 in human cells

Autor: Vladislav Kuzin, Anika Wiegard, Donald P. Cameron, Laura Baranello

Publikováno v: STAR Protocols, Vol 3, Iss 3, Pp 101581- (2022)

Summary: TOP1 CAD-seq enables mapping of TOP1 sites of covalent engagement with DNA. The procedure depends upon enrichment of DNA-covalent adducts using chaotropic salts and immunoprecipitation with an antibody specific for TOP1. Here, we describe a

Externí odkaz: https://doaj.org/article/41fd4ee2fb254065ab8c09f85533b74f

Co-inhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancerviareadthrough transcription

Autor: Donald P. Cameron, Jan Grosser, Swetlana Ladigan, Vladislav Kuzin, Evanthia Iliopoulou, Anika Wiegard, Hajar Benredjem, Sven T. Liffers, Smiths Lueong, Phyllis F. Cheung, Deepak Vangala, Michael Pohl, Richard Viebahn, Christian Teschendorf, Heiner Wolters, Selami Usta, Keyi Geng, Claudia Kutter, Marie Arsenian-Henriksson, Jens T. Siveke, Andrea Tannapfel, Wolff Schmiegel, Stephan A. Hahn, Laura Baranello

Pancreatic carcinoma is one of the most lethal cancers and the absence of efficient therapeutic strategies results in poor prognosis. Transcriptional dysregulation due to alterations in KRAS and MYC impacts initiation, development, and survival of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bf901fd90d88257c9f0696f228c9e63e
https://doi.org/10.1101/2023.02.10.527824

Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1

Autor: Roberto Ballarino, Toyoaki Natsume, Laura Baranello, Rashid Mehmood, Radosław Grochowski, Anika Wiegard, Jan Grosser, Michele Ceribelli, Donald P. Cameron, Anna H. Bizard, Fedor Kouzine, Nicola Crosetto, Vladislav Kuzin, Arne Lindqvist, Ivana Karabogdan, Francesco Valant

Publikováno v: Wiegard, A, Kuzin, V, Cameron, D P, Grosser, J, Ceribelli, M, Mehmood, R, Ballarino, R, Valant, F, Grochowski, R, Karabogdan, I, Crosetto, N, Lindqvist, A, Bizard, A H, Kouzine, F, Natsume, T & Baranello, L 2021, ' Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1 ', Molecular Cell, vol. 81, no. 24, pp. 5007-5024.e9 . https://doi.org/10.1016/j.molcel.2021.10.015

As cells enter mitosis, chromatin compacts to facilitate chromosome segregation yet remains transcribed. Transcription supercoils DNA to levels that can impede further progression of RNA polymerase II (RNAPII) unless it is removed by DNA topoisomeras

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b2285bb0b44adcf14340fca481f1e79
https://doi.org/10.1016/j.molcel.2021.10.015

Synechocystis KaiC3 displays temperature and KaiB dependent ATPase activity and is important for growth in darkness

Autor: Chihiro Azai, Anja K. Dörrich, Ilka M. Axmann, Anika Wiegard, Annegret Wilde, Kazuki Terauchi, Christin Köbler, Katsuaki Oyama

Cyanobacteria form a heterogeneous bacterial group with diverse lifestyles, acclimation strategies and differences in the presence of circadian clock proteins. In Synechococcus elongatus PCC 7942, a unique posttranslational KaiABC oscillator drives c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5106a257a994245b0d3e091805c5404

ATP synthase activity assay (radioactive) v1

Autor: Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria IM Axmann

This protocol describes how to detect synthesis of [α32P]ATP from [α32P]ATP by recombinant KaiC proteins. Radioactive nucleotides are separated via thin layer chromatography using TLC PEI Cellulose F plates as stationary phase and LiCl as soluble p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fa2caa3863acc506420e2a26ec2b5978
https://doi.org/10.17504/protocols.io.48qgzvw