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pro vyhledávání: '"Anika Raulf"'
Autor:
Alina Kollmannsperger, Armon Sharei, Anika Raulf, Mike Heilemann, Robert Langer, Klavs F. Jensen, Ralph Wieneke, Robert Tampé
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for h
Externí odkaz:
https://doaj.org/article/bd3921c263ee4105b737de9243e26428
Autor:
Kieran Finan, Mike Heilemann, Christoph Spahn, Alexander Heckel, Patrick J. M. Zessin, Anika Raulf, Stefan Bernhardt
Publikováno v:
RSC Adv.. 4:30462-30466
We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate clic
Autor:
Mike Heilemann, Robert Tampé, Anika Raulf, Robert Langer, Klavs F. Jensen, Armon Sharei, Alina Kollmannsperger, Ralph Wieneke
Publikováno v:
Nature Communications
Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
Nature
Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
Nature
Live-cell labelling techniques to visualize proteins with minimal disturbance are important; however, the currently available methods are limited in their labelling efficiency, specificity and cell permeability. We describe high-throughput protein la
Publikováno v:
Angewandte Chemie (International ed. in English). 54(41)
Techniques based on fluorescence microscopy are increasingly used to count proteins in cells, but few stoichiometrically well-defined standards are available to test their accuracy. A selection of bacterial homo-oligomers were developed that contain
Publikováno v:
Angewandte Chemie (International ed. in English). 54(35)
Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small-molecule recognition unit (
Publikováno v:
Angewandte Chemie International Edition. 54:10037-10037
Publikováno v:
Angewandte Chemie. 127:10175-10175