Zobrazeno 1 - 10
of 157
pro vyhledávání: '"Angus P. R. Johnston"'
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Measuring sub-cellular pH with high accuracy and spatiotemporal resolution remains challenging. Here, Johnston and co-workers develop a pH biosensor that combines the pH dependant fluorescent lifetime of mApple with deep learning to accurately determ
Externí odkaz:
https://doaj.org/article/1627734c9e5446319ae9a57c443160c9
Autor:
Serena L. Y. Teo, Joshua J. Rennick, Daniel Yuen, Hareth Al-Wassiti, Angus P. R. Johnston, Colin W. Pouton
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Our understanding of cytosolic delivery is hindered by existing methods for quantification which suffer from being indirect and showing low sensitivity. Here the authors report a SLEEQ (Split Luciferase Endosomal Escape Quantification) assay to asses
Externí odkaz:
https://doaj.org/article/51c2a8863f7b4866907c4bcbaa87460c
Autor:
Laura I. FitzGerald, Luigi Aurelio, Moore Chen, Daniel Yuen, Joshua J. Rennick, Bim Graham, Angus P. R. Johnston
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-loca
Externí odkaz:
https://doaj.org/article/67585245818b4c449e5738f29dc6b93c
Autor:
Gracia, Natalie L. Trevaskis, Ruby Kochappan, Enyuan Cao, Christopher Jh Porter, Angus P. R. Johnston
Publikováno v:
European Journal of Pharmaceutics and Biopharmaceutics. 172:240-252
Vaccines are a powerful health intervention but there is still an unmet need for effective preventative and therapeutic vaccines for many diseases such as cancer and infections. Interstitial (e.g. subcutaneous (SC)) injection in nano-sized carriers s
Autor:
Angus P. R. Johnston, Daniel Yuen, Colin W. Pouton, Joshua J. Rennick, Serena L. Y. Teo, Hareth Al-Wassiti
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature Communications
Nature Communications
Cytosolic transport is an essential requirement but a major obstacle to efficient delivery of therapeutic peptides, proteins and nucleic acids. Current understanding of cytosolic delivery mechanisms remains limited due to a significant number of conf
Autor:
Adelene S M, Wong, Ewa, Czuba, Moore Z, Chen, Daniel, Yuen, Kristofer I, Cupic, Shenglin, Yang, Rebecca Y, Hodgetts, Laura I, Selby, Angus P R, Johnston, Georgina K, Such
Publikováno v:
ACS macro letters. 6(3)
Targeting nanoparticles to specific cellular receptors has the potential to deliver therapeutic compounds to target sites while minimizing side effects. To this end, we have conjugated a targeting protein, holo-transferrin (holo-Tf), to pH-responsive
Autor:
Alexander P. Browning, Niloufar Ansari, Christopher Drovandi, Angus P. R. Johnston, Matthew J. Simpson, Adrianne L. Jenner
Publikováno v:
Journal of The Royal Society Interface. 19
Biological heterogeneity is a primary contributor to the variation observed in experiments that probe dynamical processes, such as the internalization of material by cells. Given that internalization is a critical process by which many therapeutics a
Publikováno v:
Australian Journal of Chemistry. 74:514-521
The use of self-assembled nanoparticles for drug delivery has received significant attention in recent years. However, the dynamic nature of self-assembled polymeric systems means there is a need to develop greater understanding of the inherent stabi
Publikováno v:
Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyREFERENCES. 14(6)
Nucleic acid therapeutics can be used to control virtually every aspect of cell behavior and therefore have significant potential to treat genetic disorders, infectious diseases, and cancer. However, while clinically approved to treat a small number
Publikováno v:
Nature Nanotechnology. 16:37-46
Induced tolerogenic dendritic cells are a powerful immunotherapy for autoimmune disease that have shown promise in laboratory models of disease and early clinical trials. In contrast to conventional immunosuppressive treatments, tolerogenic immunothe