Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Angela M. Giusti"'
Autor:
Jean-Luc Teillaud, Catherine Desaymard, Angela M. Giusti, Barbara Haseltine, Roberta R. Pollock, Dale E. Yelton, Donald J. Zack, Matthew D. Scharff
Publikováno v:
Biotechnology & Biological Frontiers ISBN: 9780429050329
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1bb598c4146035a45f83f453266660b6
https://doi.org/10.4324/9780429050329-24
https://doi.org/10.4324/9780429050329-24
Publikováno v:
Seminars in Immunology. 8:141-150
We have used conventional transgenic technology and a novel transgene homologous recombination pathway to investigate the cis-acting elements necessary for murine antibody V-gene somatic hypermutation. These studies show that an undefined element 3'
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f37dbdd2bec8e0db1dad2bec13e15957
https://doi.org/10.1006/smim.1996.0018
https://doi.org/10.1006/smim.1996.0018
Autor:
Tim Manser, Angela M. Giusti
Publikováno v:
The Journal of Experimental Medicine
We have constructed lines of mice with transgenes containing an antibody heavy (H) chain variable region (VHDJH) gene and various amounts of natural immunoglobulin (Ig) and plasmid flanking DNA. In these lines, recombination of the transgene and the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8aeee56e440ba838302b2dc37744159c
https://doi.org/10.1084/jem.179.1.235
https://doi.org/10.1084/jem.179.1.235
Publikováno v:
Proceedings of the National Academy of Sciences. 89:10321-10325
Transgenic lines of mice were derived by using plasmid constructs containing DNA encoding an antibody heavy chain variable-diversity-joining region (VH-D-JH) and various amounts of 5' and 3' flanking DNA but lacking any repetitive isotype switch (S)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20e5d5038457f59af19f05d0b9a388d6
https://doi.org/10.1073/pnas.89.21.10321
https://doi.org/10.1073/pnas.89.21.10321
Autor:
Angela M. Giusti, Lawrence P. Casson, Evongelia Notidis, Tim Manser, Kathleen M. Tumns-Btundage, Shailaja Hande, Kalpit A. Vora
Publikováno v:
Immunological reviews. 162
Somatic hypermutation and selection of immunoglobulin (Ig) variable (V)-region genes, working in concert, appear to be essential for memory B-cell development in mammals. There has been substantial progress on the nature of the cis-acting DNA element
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c7cd30f4c813df162ddbfcffe78ea1c
https://pubmed.ncbi.nlm.nih.gov/9602364
https://pubmed.ncbi.nlm.nih.gov/9602364
Autor:
Tim Manser, Angela M. Giusti
Publikováno v:
The Journal of Experimental Medicine
Mice with transgenes containing an antibody H chain V region (VHDJH) gene were used in an analysis of the cis-acting elements required for hypermutation of immunoglobulin (Ig) V genes. These transgenes can somatically recombine with endogenous IgH DN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f981b76ff15b9fe3abd43723188caa69
https://pubmed.ncbi.nlm.nih.gov/8436910
https://pubmed.ncbi.nlm.nih.gov/8436910
Autor:
Victoria A. Roberts, Nadine C. Chien, Matthew D. Scharff, Angela M. Giusti, Elizabeth D. Getzoff
Publikováno v:
Proceedings of the National Academy of Sciences. 86:5532-5536
To study the molecular basis for antibody diversity and the structural basis for antigen binding, we have characterized the loss of phosphocholine (P-Cho) binding both experimentally and computationally in U10, a somatic mutant of the antibody S107.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dad21c967866259a80e89c2a60e4cd07
https://doi.org/10.1073/pnas.86.14.5532
https://doi.org/10.1073/pnas.86.14.5532
Publikováno v:
Molecular immunology. 21(10)
BALB/c mice immunized with phosphorylcholine (PC) produce antibodies which are predominantly of the T15 idiotype. Monoclonal anti-T15 antibodies have been generated in a number of laboratories by allogenic or syngenic immunization. Most of these mous
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf745c19b32d2207f5d1bd87167586e4
https://pubmed.ncbi.nlm.nih.gov/6209566
https://pubmed.ncbi.nlm.nih.gov/6209566
Autor:
Jean Luc Teillaud, Donald J. Zack, Barbara Haseltine, Catherine Desaymard, Matthew D. Scharff, Roberta R. Pollock, Angela M. Giusti, Dale E. Yelton
Publikováno v:
Science (New York, N.Y.). 222(4625)
Hybridoma technology has made it possible to introduce into continuous culture normal antibody-forming cells and to obtain large amounts of the immunoglobulin produced by each of these cells. Examination of the structure of a number of monoclonal ant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::054f959e41dc88bda2045e412f2ba9cf
https://pubmed.ncbi.nlm.nih.gov/6356353
https://pubmed.ncbi.nlm.nih.gov/6356353
Autor:
Susan Roberts, Matthew D. Scharff, Roberta R. Pollock, Dale E. Yelton, Donald J. Zack, Angela M. Giusti, Catherine Desaymard, Sau-Ping Kwan, Pallaiah Thammana
Publisher Summary Immunoassays have been used to detect, quantitate, and localize small amounts of macromolecules in complex biological mixtures. Monoclonal antibodies are chemically defined reagents. Monoclonal antibodies have many benefits over con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1fe3e17529b3ba89159187020f96eb9e
https://doi.org/10.1016/b978-0-12-045560-7.50012-3
https://doi.org/10.1016/b978-0-12-045560-7.50012-3